Device for measuring proteins using biosensor

Abstract

A sensor for measuring a protein with a measurement speed improved from a conventional impedance measurement, using a biosensor is provided. The sensor is capable of efficiently and accurately measuring impedance generated by a selective binding to the protein by Fourier-transforming an electric current signal which is obtained by applying a potential signal of a delta function waveform.The device for measuring a protein using a biosensor includes the biosensor including a sample inlet through which a sample is drawn in, a working electrode on which a receptor layer is coated for selective binding to the specific protein in the drawn sample, and a measuring unit including a reference electrode to form a potential difference with the working electrode, a function generator which applies a potential signal in the form of delta function to the working electrode and the reference electrode, and a data processing unit which measures impedance of the working electrode by Fourier-transforming an electric current obtained in response to the delta function waveform.Accordingly, with the device for measuring a protein using a biosensor is capable of measuring concentration of the protein with accuracy, measurement time is shortened and the concentration of protein can be accurately measured by removing the influence of dispersion.

Description

TECHNICAL FIELDThe present invention relates to a device for measuring proteins, and more particularly, to a device for measuring concentration of a specific protein contained in a sample using a biosensor, in which a receptor layer optionally binding to the specific protein is formed on an electrode and impedance generated due to the binding components is measured.BACKGROUND ARTIn recent, there have been increasing demands to measure blood glucose regularly to diagnose and prevent diabetes. Portable measuring instrument, to be specific, the strip-type biosensor is available for users to measure blood glucose easily and conveniently by simply grabbing the instrument in their hands.U.S. Pat. No. 5,541,117 discloses preparing a pad with glycosylated haemoglobin assay-specific antibody fixed thereon, transferring the sample onto the fixed pad, and performing computation based on the intensity of reflected light. However, it has shortcomings in that expensive antibody is required, and s...

AI Technical Summary

Benefits of technology

To solve the above problems, the present invention provides a device for measuring protein using biosensor, in which delta function waveform is applied, and impedance of the current signals is computed by Fourier transform to efficiently and accurately measure the concentration of a specific protein in a sample.

With the device according to the present invention, measurement time is shortened and the concentration of proteins may be accurately measured.

The amount of glycated protein may be rapidly and accurately measured improving accuracy and reliability, and the device of the present invention may be available for disposable sensor.

Problems solved by technology

However, it has shortcomings in that expensive antibody is required, and sensors with consistent quality can hardly be achieved due to the inequalities of porous pad.

However, it has difficulties as it requires washing process of boronic acid derivatives, which are lysed with glycosylated protein, and a precise amount of sample should always be maintained to ensure the right results.

However, these conventional electrochemical determining methods of glycosylated protein generally involve collecting glycosylated protein and glycosylated protein-markers competitively on the surface of electrode, and injecting substrate, which stimulates electrochemical reaction with marker, to determine the size of the signal, and is thus has complicated measurement of the concentration of glycosylated protein and can hardly be reproducible.

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