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Compositions and Methods for Inhibiting Expression of Eg5 and VEGF Genes

a technology of vegf gene and compound, which is applied in the direction of biochemistry apparatus and processes, drug compositions, genetic material ingredients, etc., can solve the problems of mitotic arrest, inhibitor of eg5 which induced a transient mitotic arrest may not be effective for the treatment of cancer cell proliferation, and the lack of compounds available to control these processes within the cell, etc., to achieve the prevention of tumor growth, increase monoster formation, and reduce tumor growth

Inactive Publication Date: 2010-04-08
ALNYLAM PHARM INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0020]Also included in the invention are methods of suing the compositions of the invention. In one embodiment is are methods for inhibiting the expression of Eg5 / KSP and VEGF in a cell by administering any of the compositions of the invention to the cell. Other embodiments are methods for preventing tumor growth, reducing tumor growth, or prolonging survival in a mammal in need of treatment for cancer by administering the composition to said mammal. In some embodiments the mammal has liver cancer, e.g., the mammal is a human with liver cancer. The method can include a further step of administering Sorafenib.

Problems solved by technology

Unfortunately, there are few compounds available for controlling these processes within the cell.
These compounds alter the dynamic instability of microtubules and indirectly alter the function / structure of the mitotic spindle thereby causing mitotic arrest.
These data suggest that an inhibitor of Eg5 which induced a transient mitotic arrest may not be effective for the treatment of cancer cell proliferation.

Method used

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  • Compositions and Methods for Inhibiting Expression of Eg5 and VEGF Genes
  • Compositions and Methods for Inhibiting Expression of Eg5 and VEGF Genes
  • Compositions and Methods for Inhibiting Expression of Eg5 and VEGF Genes

Examples

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example 1

dsRNA Synthesis

[0236]Source of Reagents

[0237]Where the source of a reagent is not specifically given herein, such reagent may be obtained from any supplier of reagents for molecular biology at a quality / purity standard for application in molecular biology.

[0238]siRNA Synthesis

[0239]For screening of dsRNA, single-stranded RNAs were produced by solid phase synthesis on a scale of 1 μmole using an Expedite 8909 synthesizer (Applied Biosystems, Applera Deutschland GmbH, Darmstadt, Germany) and controlled pore glass (CPG, 500 Å, Proligo Biochemie GmbH, Hamburg, Germany) as solid support. RNA and RNA containing 2′-O-methyl nucleotides were generated by solid phase synthesis employing the corresponding phosphoramidites and 2′-O-methyl phosphoramidites, respectively (Proligo Biochemie GmbH, Hamburg, Germany). These building blocks were incorporated at selected sites within the sequence of the oligoribonucleotide chain using standard nucleoside phosphoramidite chemistry such as described in ...

example 2

Eg5 siRNA In Vitro Screening Via Cell Proliferation

[0274]As silencing of Eg5 has been shown to cause mitotic arrest (Weil, D, et al [2002] Biotechniques 33: 1244-8), a cell viability assay was used for siRNA activity screening. HeLa cells (14000 per well [Screens 1 and 3] or 10000 per well [Screen2])) were seeded in 96-well plates and simultaneously transfected with Lipofectamine 2000 (Invitrogen) at a final siRNA concentration in the well of 30 nM and at final concentrations of 50 nM (1st screen) and 25 nM (2nd screen). A subset of duplexes was tested at 25 nM in a third screen (Table 5).

[0275]Seventy-two hours post-transfection, cell proliferation was assayed the addition of WST-1 reagent (Roche) to the culture medium, and subsequent absorbance measurement at 450 nm. The absorbance value for control (non-transfected) cells was considered 100 percent, and absorbances for the siRNA transfected wells were compared to the control value. Assays were performed in sextuplicate for each o...

example 3

Eg5 siRNA In Vitro Screening Via mRNA Inhibition

[0277]Directly before transfection, HeLa S3 (ATCC-Number: CCL-2.2, LCG Promochem GmbH, Wesel, Germany) cells were seeded at 1.5×104 cells / well on 96-well plates (Greiner Bio-One GmbH, Frickenhausen, Germany) in 75 μl of growth medium (Ham's F12, 10% fetal calf serum, 100 u penicillin / 100 μg / ml streptomycin, all from Bookroom AG, Berlin, Germany). Transfections were performed in quadruplicates. For each well 0.5 μl Lipofectamine-2000 (Invitrogen GmbH, Karlsruhe, Germany) were mixed with 12 μl Opti-MEM (Invitrogen) and incubated for 15 min at room temperature. For the siRNA concentration being 50 nM in the 100 μl transfection volume, 1 μl of a 5 μM siRNA were mixed with 11.5 μl Opti-MEM per well, combined with the Lipofectamine2000-Opti-MEM mixture and again incubated for 15 minutes at room temperature. siRNA-Lipofectamine2000-complexes were applied completely (25 μl each per well) to the cells and cells were incubated for 24 h at 37° C....

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Abstract

This invention relates to compositions containing double-stranded ribonucleic acid (dsRNA) in a SNALP formulation, and methods of using the compositions to inhibit the expression of the Eg5 and Vascular Endothelial Growth Factor (VEGF), and methods of using the compositions to treat pathological processes mediated by Eg5 and VEGF expression, such as cancer.

Description

RELATED APPLICATIONS[0001]This application is a continuation of PCT Application No. PCT / US2009 / 036233, filed Mar. 5, 2009 which claims the benefit of U.S. Provisional Application No. 61 / 034,019, filed Mar. 5, 2008, and U.S. Provisional Application No. 61 / 083,367, filed Jul. 24, 2008, and U.S. Provisional Application No. 61 / 086,381, filed Aug. 5, 2008, and U.S. Provisional Application No. 61 / 112,079, filed Nov. 6, 2008, and U.S. Provisional Application No. 61 / 150,664, filed Feb. 6, 2009 which are hereby incorporated in their entirety by reference.FIELD OF THE INVENTION[0002]This invention relates to compositions containing double-stranded ribonucleic acid (dsRNA), and their use in mediating RNA interference to inhibit the expression of a combination of genes, e.g., the Eg5 and Vascular Endothelial Growth Factor (VEGF) genes formulated in SNALP, and the use of the compositions to treat pathological processes mediated by Eg5 and VEGF expression, such as cancer.BACKGROUND OF THE INVENTI...

Claims

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Application Information

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IPC IPC(8): A61K31/7088C12N5/02C12Q1/68C12N15/113
CPCC12N15/113C12N15/1136C12N2310/14C12N2310/315C12N2310/321C12N2320/31C12N2310/346C12N2310/3515C12N2310/3521A61K31/713A61P35/00A61P43/00C12N2310/11C12N2320/32
Inventor BUMCROT, DAVIDSAH, DINAH WEN-YEETOUDJARSKA, IVANKA
Owner ALNYLAM PHARM INC
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