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Model for neurodegenerative diseases involving amyloid accumulation

a neurodegenerative disease and amyloid accumulation technology, applied in the field of medical diseases, can solve the problems that many in vivo or in vitro models are unable to produce some of the important features of alzheimer's diseas

Inactive Publication Date: 2008-02-14
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a model for neurodegenerative diseases, particularly Alzheimer's disease, by creating brain cells that have enhanced sequestration, accumulation, and uptake of amyloid beta (Aβ) compared to control brain cells. This is achieved by treating brain cells with agents that modulate integrins and / or integrin receptors. The invention also provides a method for increasing the sequestration, accumulation, and uptake of Aβ in brain cells in vitro. The brain cells with enhanced Aβ levels can be used as a model for studying neurodegenerative diseases and for screening compounds that can modulate the sequestration, accumulation, and uptake of Aβ in brain cells.

Problems solved by technology

However, many in vivo or in vitro models are unable to produce some of the important features of Alzheimer's disease, such as neurofibrillary tangles, microglia activation, lysosomal dysfunction, intracellular and / or extracellular sequestration and / or uptake and / or accumulations of amyloid, etc.

Method used

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  • Model for neurodegenerative diseases involving amyloid accumulation
  • Model for neurodegenerative diseases involving amyloid accumulation
  • Model for neurodegenerative diseases involving amyloid accumulation

Examples

Experimental program
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example 1

I. Materials and Methods

[0166] A. Preparation and Maintenance of Hippocampal Slice Cultures

[0167] Organotypic hippocampal cultures were prepared using the technique of Stoppini et al. (1991). Briefly, hippocampi were harvested from brains of 9-12 days old Sprague-Dawley rat pups under sterile conditions. Sections were cut (400 μm thick) perpendicular to the long axis of hippocampus using a McIllwain tissue chopper and were collected into a cutting medium consisting of MEM with Earle's salts (Gibco, Rockville Md.), 25 mM HEPES, 10 mM Tris base, 10 mM glucose, and 3 mM MgCl2 (pH 7.2). Slices were positioned onto 30 mm cell culture inserts (Millicell-CM, Millipore, Bedford, Mass.) that were placed in 6 well culture trays with 1 ml of growth medium per well [growth medium: MEM with Hank's salts (Gibco), 20% horse serum, 3 mM glutamine, 25 mM HEPES, 5 mM NaHCO3, 25 mM glucose, 0.5 mM ascorbate, 2 mM CaCl2, 2.5 mM MgCl2, 0.5 mg / l insulin, and penicillin, pH 7.2]. The cultures were incub...

example 2

[0200] An embodiment of the invention drawn to a pharmaceutical composition and the use of that composition to treat neurodegenerative diseases such as Alzheimer's disease. The composition alleviates the symptoms of characteristics associated with Alzheimer's disease such as intracellular uptake of amyloid protein, amyloid accumulation or plaque formation,

[0201] A patient in need of intervention for Alzheimer's disease is selected based on currently used diagnostic guidelines and evaluation criteria such as those detailed: by the National Institute of Neurological and Communicative Disorders and Stroke-Alzheimer's Disease and related Disorders Association (NINCDS-ADRDA), the Alzheimer's Disease section found in the Diagnostic and Statistical Manual of Mental Disorders 4th edition (DSM-IV), by the National Institute of Neurological Disorders and Stroke and the Association pour la REcherche et l'Enseignement en Neurosciences (NINDS-AIREN), and / or by the California Alzheimer's Disease...

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Abstract

The present invention provides brain cells, such as normal brain cells, apolipoprotein E deficient brain cells, or apoE4 containing brain cells, that are treated with a compound which can modulate integrins and / or integrin receptors to produce increased sequestration of and / or accumulation of and / or uptake of Aβ, and / or changes in cathepsin D content and / or lysosomal dysfunction, and / or microglia activation in the brain cells. The present invention also provides methods for producing such cells and methods for using the cells for screening an agent or substance that modulates the sequestration of and / or accumulation of and / or uptake of Aβ, and / or lysosomal dysfunction, and / or changes in cathepsin D content and / or microglia activation in the brain cells. The method further provides a new therapeutic target, antagonism of glutamate receptors, for the treatment of neurodegenerative diseases which are characterized by inter alia, abnormal amyloid uptake and / or accumulation.

Description

CROSS-REFERENCES TO RELATED APPLICATIONS [0001] This application claims the benefit of U.S. Provisional Application No. 60 / 235,374 filed Sep. 25, 2000, the contents of which is incorporated herein by reference in its entirety.STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT [0002] This invention was made with Government support under Grant No. 455365-30110, awarded by the National Institute of Aging. The Government may have certain rights in this inventionFIELD OF THE INVENTION [0003] The invention is in the field of models and interventions of medical diseases. Specifically, the invention is in the field of neurodegenerative disease models and treatments, and especially age related neurodegenerative diseases such as Alzheimer's disease. BACKGROUND OF THE INVENTION [0004] Alzheimer's disease (AD) is a leading cause of dementia in the elderly, affecting 5-10% of the population over the age of 65 years. See A Guide to Understanding Alzheimer...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/13A61K31/135A61K31/352A61K31/40A61K31/44A61K31/445A61K31/485A61K31/55A61K33/06A61K38/07A61K38/08A61K38/17C12N5/00C12Q1/02G01N33/53C07K14/775C12N15/85C12Q1/00C12Q1/68G01N33/50G01N33/68
CPCA01K2207/15A01K2217/00A01K2217/05A01K2217/075A01K2227/105G01N2800/52A01K2267/0318C07K14/775C12N15/8509G01N33/5058G01N33/6896A01K2267/0312
Inventor LYNCH, GARYBI, XIAONINGGALL, CHRISTINE M.
Owner RGT UNIV OF CALIFORNIA
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