Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto

a technology of surface assembly and dna capture probe, which is applied in the field of detection of specific sequences of nucleic acids or oligonucleotides, can solve the problems of not being easily adapted to macromolecules, such as antibodies and dna

Inactive Publication Date: 2006-04-13
VINDUR TECH
View PDF26 Cites 39 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"This patent describes a method for identifying specific DNA or RNA sequences in a sample using a bio disc with a flow channel and a detection system. The bio disc can be made using an optical disc and can include information specific to the DNA or RNA test being conducted. The detection system uses a laser to interrogate the sample and process the signals. The method can be performed using a transmissive or reflective disc format. The patent also describes a method for attaching enzymes to the disc surface for use in the detection process. Overall, the patent provides a reliable and sensitive method for identifying specific DNA or RNA sequences."

Problems solved by technology

This format is very useful for the detection of small molecules, but is not easily adapted to macromolecules, such as antibodies and DNA.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto
  • Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto
  • Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto

Examples

Experimental program
Comparison scheme
Effect test

experimental examples

[0166] Having generally described the invention, the same will be more readily understood through reference to the following examples, which are provided by way of illustration, and are not intended to be limiting of the present invention.

example 1

Spin Coating the Bio-disc

[0167] Fresh polystyrene solution was prepared by adding 3 g polystyrene pellets (Sigma cat. no. 182427; molecular weight=280,000) to 310 ml toluene and stirring for 1 hour using a teflon stir bar and a stir plate. After the polystyrene was completely dissolved in the toluene, 68 ml reagent grade isopropanol was slowly added while stirring.

[0168] A stock solution of nitrocellulose was prepared by diluting a nitrocellulose collodium solution (4-8% in ethanol / diethylether, Fluka cat. no. 09986, lot no. 389973 / 1 30299) 1:5 in reagent grade ethanol. Prior to spin coating, the stock solution was diluted 1:10 with reagent grade ethanol and filtered using a 0.2 μm syringe filter.

[0169] A polycarbonate disc having a 200 Angstrom gold semi-reflective layer (BTI Optical Bio-disk Set FDL21:E001308) was placed on a “spin coater,” or modified centrifuge, with the reflective surface up. While rotating the disc on the spin coater, the reflective surface was cleaned with...

example 2

Preparing the Bio-disk for the Enzyme Assay

[0172] The disc from Example 1 was placed on a CD assembler / spindle with the nitrocellulose layer up. Between about 0.5 to 2.0 μl of 1 μM oligo probes (capture DNA) in 1 M NH4OAc were applied to the disk at defined target zones. The droplets of capture DNA were dried onto the nitrocellulose at 37□C.

[0173] A cover disc containing U-shaped fluidic circuits (50 μM adhesive; Fraylock, DBL243a) was applied using a disk assembler spindle, and the disc was run through a wringer to seal the two disks.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thickaaaaaaaaaa
thickaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention relates to methods and systems for use of bio-discs in quantifying an amount or concentration of one or more substances that are present in a biological sample. The system includes an optical disc having one or more samples deposited thereon, an optical element configured to emit and divert radiation onto the samples and components for measuring spectral change of the compound and determining an amount or concentration of one or more target substances. The bio-disc is prepared using a method including providing a membrane that is or can be dimensional to fit within a channel of the bio-disc, applying a reagent on the membrane which is configured to allow the reagent to be released from the membrane into a solution in contact with the membrane, and depositing the membrane in a channel of the bio-disc.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims the benefit of U.S. Provisional Applications Ser. No. 60 / 292,110, filed May 18, 2001, and Ser. No. 60 / 313,917, filed Aug. 21, 2001, and is a continuation of U.S. application Ser. No. 10 / 150,702, filed May 17, 2002, the contents of which are hereby incorporated by reference in their entireties.BACKGROUND OF THE INVENTION [0002] The present invention relates to methods and systems for the detection of specific sequences of nucleic acids or oligonucleotides, including deoxyribonucleic acids (DNA) and ribonucleic acids (RNA). It relates more particularly to a conjugated enzyme based assay system utilizing reflective and / or transmissive optical discs for detection of specific sequences of nucleic acids. [0003] Assay systems utilizing optical discs have been described. See, for example, Virtanen, U.S. Pat. No. 6,030,581 entitled “Laboratory in a Disk”. Such systems have enormous potential in the field of medicine, for ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68G01N1/28C12M1/34B05D3/00B01J19/00B01L3/00C40B40/06C40B50/14C40B60/14G01N35/00
CPCB01J19/0046B01J2219/00274B01J2219/00317B01J2219/005B01J2219/00536B01J2219/00576B01J2219/00578B01J2219/00585B01J2219/00596B01J2219/00605B01J2219/0061B01J2219/00617B01J2219/00621B01J2219/0063B01J2219/00637B01J2219/00641B01J2219/00648B01J2219/00659B01J2219/00675B01J2219/00689B01J2219/00695B01J2219/00702B01J2219/00707B01J2219/00722B01J2219/00725B01L3/5025B01L3/5027B01L3/502707B01L3/502753B01L3/545B01L2300/0806B01L2400/0409B82Y30/00C40B40/06C40B50/14C40B60/14G01N35/00069Y10S977/924
Inventor WERNER, MARTIN ELISABETHVALENCIA, RAMON MAGPANTAYGORDON, JOHN FRANCIS
Owner VINDUR TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com