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Methods and compositions for genetically detecting improved milk production traits in cattle

a technology of milk production traits and compositions, applied in the field of cattle progeny testing, can solve the problems that selection based purely on phenotypic characteristics does not efficiently take into account genetic variability, and achieves low somatic cell score, high milk protein percentage, and high production life.

Inactive Publication Date: 2005-06-09
WISCONSIN ALUMNI RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0018] The present invention also provides a method for testing a dairy cattle for its milk production trait, comprising haplotyping its cells, wherein a cattle having haplotype 1, 3, 4 or 5 indicates that the cattle has desirable milk production trait. Preferably, the test is for a cattle having haplotype 1 which indicates that the cattle has desirable milk production trait, health and reproduction traits. Haplotype 1 is associated with high milk protein percentage, high productive life, low somatic cell score, and high daughter pregnancy rate. Haplotype 3 is associated with milk fat. Haplotype 4 is associated with high milk yield, high somatic cell score and low daughter pregnancy rate. Haplotype 5 is associated with high milk yield, low fat percentage, low protein percentage, high somatic cell score and low daughter pregnancy rate. Thus it would be desirable to make selection decisions on haplotype 1 that does not show any negative effects.

Problems solved by technology

Furthermore, selection based purely on phenotypic characteristics does not efficiently take into account genetic variability caused by complex gene action and interactions, and the effect of the environmental and developmental variants.

Method used

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  • Methods and compositions for genetically detecting improved milk production traits in cattle
  • Methods and compositions for genetically detecting improved milk production traits in cattle

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example 1

Identification of Haplotypes and Determination of Their Association with Milk Production Traits

Resource Population and Phenotypic Data

[0055] The Cooperative Dairy DNA Repository (CDDR) is an extension of the Dairy Bull DNA Repository (DBDR) started at the University of Illinois in 1993. The DBDR was established to identify QTL in large Holstein families using the granddaughter design (Weller et al. 1990). In the granddaughter design, QTL are mapped using genotypes from grandsires and their sons and the granddaughters' phenotypic values as the trait endpoints. QTL can be mapped and markers flanking the QTL can be identified. Once flanking markers have been identified, marker-assisted selection can be utilized to shorten the generation interval and reduce time and cost of progeny testing.

[0056] Thirty seven (37) half-sib families comprised of 2,363 sons were selected from the CDDR collection for quantitative trait gene (QTG) detection using the granddaughter design. Data for Predi...

example 2

Experimental Design for Identification of Haplotypes

[0078] The following is exemplifies experimental designs for determining haplotypes of a sample. Genomic DNA from the sample may be first amplified via PCR with primers PI9, PI10 followed with restriction enzyme RsaI. This enzyme digests C allele only at position 989, so that TT products would not be digested, while CT and CC products would be digested.

[0079] Haplotype 1 can then be differentiated from 2 and 3 by specific primer amplification at position 164, by designing a primer that ends with A or G.

[0080] Haplotype 2 can be differentiated from 3 by specific primer amplification at position 269 or 285.

[0081] Haplotype 6 can be differentiated from 4 and 5 by position 164.

[0082] Haplotype 4 can be differentiated from 5 by positions 269 and 285.

TABLE 3Comparison of Various Traits Between Haplotype 1 (TT) and Other HaplotypesANOVAContrastRegressionanalysisMean / genotypeanalysisanalysisTraitPTTCTCCTT vs. othersCC vs. otherspPTA...

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Abstract

An isolated nucleic acid molecule comprising a polymorphic site selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1, an array or a kit comprising the same. Also provided are a method for detecting single nucleotide polymorphism (SNP) in bovine proteinase inhibitor (PI) gene, a method for haplotyping a bovine cell, a method for progeny testing of cattle based on said haplotyping, a method for selectively breeding of cattle based on haplotyping a parent animal. The present invention further provides a method for testing a dairy cattle for its milk production trait, comprising haplotyping its cells, wherein a cattle having haplotypes 1, 3, 4 or 5 indicates that the cattle has desirable milk production trait. Haplotype 1 indicates that the cattle has the most desirable milk production trait.

Description

[0001] This invention was made with United States government support awarded by USDA / CSREES, under the grant number 05-CRHF-0-6055. The United States may have certain rights in this application.FIELD OF THE INVENTION [0002] The present invention relates to a method of cattle progeny testing using molecular genetic methods by assaying for the presence of at least one genetic marker which is indicative of improved milk production and reproduction traits, including milk yield and milk composition, somatic cell score, productive life, and daughter pregnancy rate. BACKGROUND OF THE INVENTION [0003] Dairy cows are significant investments for dairy farmers, and enormous efforts, such as animal breeding and artificial insemination, have been and continue to be invested in ensuring that the animals have high and sustained productivity, and that the milk produced are of high quality. A successful breeding family is the Holstein line derived from Carlin-M Ivenhoe Bell. More than 25% of the hig...

Claims

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Application Information

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IPC IPC(8): A01K67/027C07H21/04C12N
CPCC12Q1/6876C07H21/04A61D19/02A61D19/04C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor KHATIB, HASAN
Owner WISCONSIN ALUMNI RES FOUND
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