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Method for promoting electroporation efficiency utilizing dielectrophoresis for assisting cell positioning

A technology of dielectrophoresis and assisting cells is applied in the field of using dielectrophoresis to assist cell positioning and biochips, and can solve problems such as low electroporation efficiency.

Inactive Publication Date: 2007-07-11
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The present invention uses cell dielectrophoresis to improve the efficiency of in situ cell electroporation on traditional microfluidic biochips, in order to improve the situation of low electroporation efficiency caused by the fact that cells cannot be positioned in the area of ​​effective electric field strength. Purpose

Method used

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  • Method for promoting electroporation efficiency utilizing dielectrophoresis for assisting cell positioning
  • Method for promoting electroporation efficiency utilizing dielectrophoresis for assisting cell positioning
  • Method for promoting electroporation efficiency utilizing dielectrophoresis for assisting cell positioning

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Embodiment Construction

[0030] The technical solution of the present invention is to process electrodes or electrode arrays on a microfluidic chip by using micromachining technology. The electrodes include two groups. The first group is used to generate dielectrophoresis force for cell manipulation and cell positioning. The second group Used for cell electroporation. Before cell electroporation, first add the cell culture medium or other buffer solutions to the microfluidic chip, and then apply a certain frequency and amplitude voltage to the first set of electrodes (the frequency and amplitude depend on the cell's medium The electrical properties vary with different cell types), prompting cells to concentrate in the effective area of ​​the designed electroporation.

[0031] At this time, there are two technical routes to choose from. First, you can directly apply electroporation voltage to electroporate cells that are still in suspension; second, you can allow cells to grow in situ for a period of time ...

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Abstract

The invention discloses an improving method of electric punching efficiency located by dielectric electrophoretic auxiliary cell, which is characterized by the following: locating the cell; exerting electricity to pierce cell; improving the punching efficiency; controlling electric field to operate cell; integrating on the micro-flow biological chip; realizing full-automatic cell operation and electric punching operation.

Description

Technical field [0001] The invention relates to a biochip, particularly in the field of microfluidic chips, and is a method for improving cell electroporation efficiency by using dielectrophoresis to assist cell positioning. The present invention uses cell dielectrophoresis to improve the efficiency of in-situ cell electroporation on the traditional microfluidic biochip, in order to improve the situation of low electroporation efficiency due to the cells cannot be positioned in the area of ​​effective electric field strength purpose. Since the method adopts a method of using a controlled electric field to manipulate cells, it is very beneficial for microsystem integration on a microfluidic biochip, and can realize fully automated operation. Background technique [0002] In the fields of life sciences and medicine, gene transfer technology is receiving more and more attention. The introduction of foreign genes, proteins, drugs, etc. into cells (ie transfection) has very important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87
CPCC12M33/00C12M35/02C12N13/00C12M23/16
Inventor 王磊郭旻程京
Owner CAPITALBIO CORP
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