Nucleic acid sequence of wild emmer alpha-amylase inhibiting factor and its application

Abstract

The present invention discloses coding gene sequence and amino acid sequence of wild emmer alpha-amylase inhibiting factor, and belongs to the field of biological gene engineering technology. The sequences are shown in SEQ ID Nos. 1-6 of the sequence list. The gene sequence may be introduced via different methods to wheat to culture excellent wheat breed with high pest resistance. In the same time, specific convenient molecular marker system may be established based on the cloned gene sequence for directive breeding. Furthermore, yeast expression vector containing the alpha-amylase inhibiting factor coding gene may be established and expressed in yeast to obtain alpha-amylase inhibiting factor, which is used as botanical pesticide added into common pesticide product.

Description

technical field [0001] The invention belongs to the technical field of biogenetic engineering, and in particular relates to a nucleic acid sequence of a gene encoding a wheat α-amylase inhibitor and the application of the sequence to improve insect resistance of wheat through biotechnological methods. Background technique [0002] Due to the attack of various pests, the world's food production will be reduced by 37% every year. The in-depth research and wide application of chemical insecticides have achieved remarkable insect control effects in production, but at the same time, the cost of chemical insecticides to control insect pests is high, the environment is polluted, and it is easy to cause insecticide resistance and ecological balance imbalance. Therefore, in recent years, the focus of research has been on how to improve the insect resistance of plants, and the breeding for the introduction of heterologous (plant, animal, microorganism) insect resistance genes has been...

AI Technical Summary

Problems solved by technology

The 24kDa wheat dimeric α-amylase inhibitor (WDAI) is one of the natural insect-resistant proteins, which can inhibit the α-amylase of certain pests. The inhibitors in this protein family all have 124 amino acid residues The two factors of WDAI-0.19 and WDAI-0.53 have only 7 substitutions in the amino acid sequence, and they have 94% homology. Insect α-amylase, there is also a 100-fold difference in the inhibitory activity of the same amylase

However, there is no research on the structural and functional differences between different inhibitors at the gene level, and the inhibitory effect on amylases from different sources, so it is impossible to select a more suitable inhibitor gene for transgenic work