Fluorescence PII sensor with inner reference and comparison, and preparation method

Abstract

A fluorescent pH transducer with internal reference consists of slide being processed by silanization, porphyrin derivative and benzo thiaanthracence derivative. The method for preparing fluorescent pH transducer with internal reference is also disclosed.

Description

technical field [0001] The invention relates to a sensor and a preparation method thereof, in particular to a fluorescent pH sensor with an internal reference and a preparation method thereof. Background technique [0002] The determination of pH value is very important in various scientific researches and practical applications, and the development of pH sensors has also attracted much attention. At present, the most widely used pH sensor is the glass electrode pH sensor, but it has high impedance, is easy to be damaged, and has defects such as "sodium error" in the case of high alkalinity. [0003] The optical fiber pH sensor has greatly improved the deficiency of the glass electrode pH sensor. In the research of the optical fiber pH sensor, due to the inherent sensitivity and selectivity of the fluorescence method, the richness of the fluorescence measurement signal and the simplicity of the design, the sensor based on the fluorescence principle Research is extremely act...

AI Technical Summary

Problems solved by technology

However, there are few such pH-sensitive fluorescent dyes with dual wavelengths at present; the other is to use two fluorescent dyes, namely, a sensitive dye that is sensitive to pH changes and a reference dye that is insensitive to pH changes to achieve a fluorescence ratio. Measurement

Although there are many kinds of known pH-sensitive dyes, most of the reported literatures are limited to fluorescein derivatives as sensitive dyes.

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