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Preparation method of wheat germ agglutinin and its application in inhibiting mammary gland cancer activity

A wheat germ agglutinin and wheat germ technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and medical preparations containing active ingredients, etc., to achieve the effects of scientific and reasonable process design, no environmental pollution, and improved utilization value.

Inactive Publication Date: 2007-04-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its preparation process can be further improved, and its application needs to be further developed.

Method used

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  • Preparation method of wheat germ agglutinin and its application in inhibiting mammary gland cancer activity
  • Preparation method of wheat germ agglutinin and its application in inhibiting mammary gland cancer activity
  • Preparation method of wheat germ agglutinin and its application in inhibiting mammary gland cancer activity

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Take 500g of fresh wheat germ, grind it through a 20-mesh sieve, degrease with 2L of pre-cooled petroleum ether (bp 40-60°C) twice, and degrease with 2L of pre-cooled acetone twice, and let the solvent evaporate naturally at room temperature until the smell disappears. After grinding, pass through a 60-mesh sieve, use 9 times 0.5M formic acid solution, ultrasonic intensity 30W / cm2, stir and extract at room temperature for 0.5h, and then centrifuge at 4°C, 25000g for 20min. The supernatant was salted out with 40% saturation of ammonium sulfate, overnight at 4°C, and centrifuged at 25000g for 20min at 4°C. The precipitate was dissolved in 500 mL of water, heat-treated at 65°C for 10 minutes, centrifuged at 25,000 g for 20 minutes at 4°C, and the precipitate was removed to obtain the supernatant. Ultrafiltration of the supernatant: first use a hollow fiber ultrafiltration tube with a molecular cut-off value of 50kD of 2.5cm×20cm for ultrafiltration, and the ultrafiltration...

Embodiment 2

[0032] Take 500g of fresh wheat germ, grind it through a 20-mesh sieve, degrease with 2L of pre-cooled petroleum ether (bp 40-60°C) twice, and degrease with 2L of pre-cooled acetone twice, and let the solvent evaporate naturally at room temperature until the smell disappears. After grinding, pass through a 60-mesh sieve, use 11 times 0.3M formic acid solution, ultrasonic intensity 30W / cm2, stir and extract at room temperature for 1.5h, and then centrifuge at 4°C and 25000g for 20min. The supernatant was salted out with 40% saturation of ammonium sulfate, overnight at 4°C, and centrifuged at 25000g for 20min at 4°C. The precipitate was dissolved in 500 mL of water, heat-treated at 65°C for 10 minutes, centrifuged at 25,000 g for 20 minutes at 4°C, and the precipitate was removed to obtain the supernatant. Ultrafiltration of the supernatant: first use a hollow fiber ultrafiltration tube with a molecular cut-off value of 50kD of 2.5cm×20cm for ultrafiltration, and the ultrafiltra...

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Abstract

This invention relates to the preparation of wheat germ agglutinin (WGA) and its application in restraining breast cancer's activity. It belongs to a field of wheat by-product material's deep processing technology. The wheat germ was comminuted, degreased,deep comminuted, super sound intensified acid extracted, salting out, heat treated, ultrafiltrated and affinity chromatographed, then the composition which have red cell agglutinating activity was desalted and freeze dried, and at last WGA is gained. Cell culture determines that the WGA has depressant effect on rat breast cancer cell strain SHZ and trypsin dependent form human breast cancer cell strain MCF-7. This invention's technology design is scientific and reasonable, manipulate is convenient and procedure is not polluted. The invention provides groundwork for exploiting drug of restraining cancer, and can enhance the utilization value of wheat germ deep processing and related industry's economic returns.

Description

technical field [0001] A preparation method of wheat germ agglutinin and its application in inhibiting breast cancer activity belong to the technical field of intensive processing of wheat by-products. Background technique [0002] Wheat germ is a by-product of wheat processing, with an annual output of more than 2 million tons. This is a considerable resource, which is worth developing and utilizing. Wheat Germ Agglutinin (WGA) is a highly conserved chitin-binding lectin. Although WGA has a small content in grains, it has various important biological activities, especially tumor suppressor activity. However, for a long time, my country's wheat germ processing has only been in a state of primary processing that satisfies human and animal nutrition. The structure of processed products is single, the level of comprehensive utilization is low, and the level of deep processing is far lower than that of developed countries. [0003] Using wheat germ as raw material, wheat germ a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14A61K38/00A61P35/00
Inventor 单良姚惠源张晖陈正行
Owner JIANGNAN UNIV
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