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Bacillus alcaligenes and its application in preparing Weilan gum

A technology of Alcaligenes and welan gum, which is applied in the field of fermentation engineering, can solve the problems of no welan gum and other problems, and achieve the effects of reduced alcohol solvent consumption, good temperature tolerance, and reduced alcohol solvent consumption

Inactive Publication Date: 2007-01-03
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production of welan gum mainly adopts the microbial fermentation method. The U.S. kelco company screened a welan gum producing bacterium with the preservation number ATCC31555, and realized the industrial production of welan gum with this bacterium as the production strain. See other related reports on welan gum production

Method used

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  • Bacillus alcaligenes and its application in preparing Weilan gum

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Experimental program
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Effect test

Embodiment 1

[0053] Incline medium: peptone 10g / L, beef extract 3g / L, NaCl 5g / L, agar 20g / L, pH7.2.

[0054] Shake flask medium: glucose 50g / L, bean cake powder 4g / L, peptone 1g / L, K 2 HPO 4 2g / L, MgSO 4 0.1g / L, pH7.2. Fill a 250ml Erlenmeyer flask with a volume of 50ml, and sterilize at 121°C for 15 minutes.

[0055] Cultivate Alcaligenes NXG-3 with the preservation number of CGMCC No.1737 on a slant medium at 28-32°C for 72 hours, then put a ring of this bacteria in the shake flask medium, culture at 30°C for 72 hours, and shake the flask at a rotational speed of 200r / min, the content of welan gum in the fermented liquid obtained at last is 18g / L.

Embodiment 2

[0057] Glucose 175g, peptone 14g, K 2 HPO 4 7g, MgSO 4 0.35g, pH7.2, dilute to 3.5L with water, make fermentation broth, put into a 7.5L glass stirring fermenter, and steam sterilize at 121°C for 15 minutes. Preparation of seed medium: glucose 20g / L, peptone 4g / L, yeast extract 1g / L, K 2 HPO 4 2g / L, MgSO 4 0.1g / L, pH7.2. Inoculate two rings of Alcaligenes NXG-3 with the preservation number CGMCC No.1737 in the seed culture medium, cultivate at 30°C for 16 hours to obtain the seed liquid, and insert the seed liquid into the cooled fermentation liquid according to the inoculation amount of 10% of the fermentation liquid volume In the fermentation broth, cultured at 30°C (ventilation rate 1vvm, stirring speed 600r / min) for 60h, the fermentation broth contained 27g / L of welan gum, and the viscosity reached 8000cp.

Embodiment 3

[0059] Mix 1750 grams of cornstarch with an appropriate amount of water, add an appropriate amount of high-temperature α-amylase solution, and add 140 g of bean cake powder at the same time, K 2 HPO 4 70g, MgSO 4 3.5g, pH 7.2, dilute to 35L with water, put into a 50-liter mechanically stirred fermenter, heat at 90°C for 10 minutes for liquefaction, then heat up to 121°C for 10 minutes for steam sterilization. Prepare seed culture medium, formula and culture method are the same as embodiment 2, insert the seed liquid into the fermented liquid after cooling according to the inoculum amount of 10% of the fermented liquid volume, cultivate at 30 ℃ for 60 hours, and the mature fermented liquid is warmed up to 80~90 ℃ inactivation for 15 minutes, after cooling, add 95% ethanol with twice the concentration of the fermentation broth, keep it overnight at 4 ℃, filter and remove the supernatant, and dry the precipitate in a vacuum oven at 55 ℃ for about 3 hours to obtain Finished We...

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Abstract

The present invention discloses one kind of Bacilus alcaligenes and its application in preparing Weilan gum. The Bacilus alcaligenes strain has the preservation number of CGMCC No. 1737. The process of preparing Weilan gum with the Bacilus alcaligenes strain includes inoculating the strain to bacteria-free culture medium containing carbon source, nitrogen source, inorganic salt and water; ventilating and stirring culturing in proper conditions; and extracting the fermented liquid to obtain Weilan gum. The Bacilus alcaligenes strain can utilize several kinds of carbon source and nitrogen source and has extensive culture conditions, and the process is simple and has high Weilan gum yield, high Weilan gum quality and low production cost.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and relates to a microbial strain Alcaligenes.sp. NXG-3 with high yield of Welan gum, and also relates to the application of the bacteria in preparing Welan gum. Background technique [0002] Over the past half century, microbial polysaccharides have been greatly developed, especially in the 1960s, xanthan gum products were commercialized for the first time, which aroused great interest in the scientific and industrial circles. Subsequently, new types of microbial polysaccharides emerged in an endless stream. Welan gum is a heterogeneous polysaccharide copolymer synthesized by microorganisms, and its monomer composition is glucose, glucuronic acid, mannose and rhamnose. Welan gum has many excellent and unique physical and chemical properties. It can be widely used in petroleum, concrete, and paint industries as a thickener, suspending agent, and stabilizer. It can also be used i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/02C12R1/05
Inventor 徐虹李莎郭朝江欧阳平凯
Owner NANJING UNIV OF TECH
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