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Cell culturing rack material and its prepn

A scaffold material and cell culture technology, applied in the direction of coating, etc., can solve the problems of unfavorable cell growth and culture, low porosity, poor penetration of the upper and lower pores, etc., to achieve the best tissue compatibility, overcome poor strength, and good growth environment Effect

Inactive Publication Date: 2007-01-03
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Chinese invention patent "A Porous Silk Fibroin Film and Its Preparation Method" with the publication number CN1260363A discloses a porous silk film, which uses silk as the main film-forming raw material, and adopts vacuum freeze-drying to prepare the porous silk fibroin film. The film thickness, porosity is low, and the pores are poorly connected up and down, which is not conducive to the growth and cultivation of cells.

Method used

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  • Cell culturing rack material and its prepn
  • Cell culturing rack material and its prepn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Put 0.2kg of waste mulberry silk into 6L of 0.05% sodium carbonate aqueous solution, boil for 0.5h, repeat the treatment three times, remove all the sericin around the silk, obtain silk fibroin, and dry at room temperature;

[0027] 2. Dissolve the dried silk fibroin with 1.2L of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 78±2°C to form a silk fibroin solution;

[0028] 3. Pour the silk fibroin solution prepared in step 2 into a cellulose dialysis bag, first dialyze with tap water, then dialyze with deionized water to remove ethanol and calcium chloride small molecules in the solution, and then use multi-layer degreasing Filter through gauze to obtain pure silk fibroin solution;

[0029] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​20×20cm 2 In a polystyrene plastic tray, dry and form a film at constant temperature and humidity (25°C, RH65%), take off the regenerated silk fibroin film and pack it into a b...

Embodiment 2

[0036] 1. Put 0.1kg of waste silk into 3L of 0.05% sodium carbonate aqueous solution, boil for 0.5h, repeat the treatment three times, remove all the sericin around the silk, and obtain silk fibroin;

[0037] 2. Dissolve the dried silk fibroin obtained in step 1 with 0.6 L of calcium chloride, water, and ethanol solution with a molar ratio of 1:8:2 at 78±2° C. to form a silk fibroin solution;

[0038] 3. Pour the above-mentioned silk fibroin solution into a cellulose dialysis bag, dialyze with tap water first, and then dialyze with deionized water to remove ethanol and small calcium chloride molecules in the solution, and then filter with multi-layer degreasing gauze to prepare Obtain pure silk fibroin solution;

[0039] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​20×20cm 2 In a stainless steel plate, freeze at -20°C for 8 hours, and then vacuum-dry in a freeze dryer for 20 hours to obtain a spongy regenerated silk fibroin film.

[0040] 5. Weig...

Embodiment 3

[0046] 1. Put 0.2kg of waste mulberry silk into 6L of 0.05% sodium carbonate aqueous solution, boil for 0.5h, repeat the treatment three times, remove all the sericin around the silk, obtain silk fibroin, and dry at room temperature;

[0047] 2. Dissolve the dried silk fibroin with 1.2L of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 78±2°C to form a silk fibroin solution;

[0048] 3. Pour the silk fibroin solution prepared in step 2 into a cellulose dialysis bag, first dialyze with tap water, then dialyze with deionized water to remove ethanol and calcium chloride small molecules in the solution, and then use multi-layer degreasing Filter through gauze to obtain pure silk fibroin solution;

[0049] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​10×20cm 2 In a polystyrene plastic tray, dry and form a film at constant temperature and humidity (25°C, RH65%), take off the regenerated silk fibroin film and pack it into a b...

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Abstract

The present invention discloses one kind of cell culturing rack material and its preparation process. The cell culturing rack material is prepared with silk as main material, and through degumming, dissolving, purifying and drying to form fimbrin; dissolving together with collagen or gelatin in the same kind of solvent; high voltage electrostatic spinning to obtain 3D netted nanometer fiber non-woven felt of fimbrin base material, collagen, gelatin, etc. and final organic alcohol treatment. The cell culturing rack material has average fiber diameter smaller than 100 nm, average pore size of 1.0-5.0 microns and porosity of 70-90%. Test shows that the nanometer fiber material has no toxicity, no irritation, no sensibilization, excellent tissue compatibility and perforated pore structure, and may be used as cell culturing rack material for repairing human body tissue.

Description

technical field [0001] The invention relates to a medical biological material and a preparation method thereof, in particular to a nanofiber non-woven material which uses a silk fibroin layer as a base material and is compounded with collagen, gelatin or a mixture of collagen, gelatin and silk fibroin. The invention relates to a felt-structured cell culture support material and a preparation method thereof, which belong to the technical field of polymer materials. Background technique [0002] Before the present invention was made, natural or synthetic high polymers were used to make nanofiber non-woven membranes through electrospinning as cell culture carriers, such as: PCL, PLA, PLGA, collagen, etc., the first three are hydrophobic, In the hydrophilic biological environment, it is not conducive to the adhesion of cells; the latter is a hydrophilic natural polymer, which is conducive to the adhesion and growth of cells, but due to poor spinnabili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34
Inventor 张幼珠尹桂波鲍韡韡王曙东吴佳林
Owner SUZHOU UNIV
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