Method for preparing laver amylose

Abstract

This invention provides a method for preparing porphyra polysaccharides. The method comprises: (1) pulverizing dry porphyra, and refluxing with ethanol to remove the fat; (2) adding 50 times of water with pH of 8, ultrasonicating for 30 min, boiling in a constant temperature water bath of 100 deg.C for twice, incorporating the extracts, and centrifuging to remove the impurities; (3) concentrating to half of the original volume, adding into an organic solution at a volume ratio of 5:1, vigorously shaking at 350 rpm for 30 min, and centrifuging at 500 rpm for 6 times (10 min for each time); (4) concentrating the supernatant to 1 / 3 of the original volume, adding 4 times of ethanol, standing at 4 deg.C for 12 h, and centrifuging to obtain polysaccharides precipitate; (5) washing with acetone, and freeze-drying in vacuum to obtain the final product.

Description

Technical field: [0001] The invention relates to a method for preparing economically valuable substances from seaweed, in particular to a method for preparing laver polysaccharides. Background technique: [0002] Porphyra polysaccharides have multiple biological functions. At present, a lot of research is being carried out on its biological activities. It was found that Porphyra polysaccharides have lectin-like effects and participate in the activation of phagocytes and biological defense; different components of Porphyra polysaccharides have different effects, polysaccharide PY3 is an immune enhancer, and polysaccharides PY2 and PY4 are immunosuppressants , can be used for the treatment of clinical hyperimmunity; it has the effect of immune regulation. It can reduce the content of serum total cholesterol and triglyceride in rats with hyperlipidemia, and has the effect of preventing the formation of hypercholesterolemia in mice caused by high cholesterol, and has a certain ...

AI Technical Summary

Problems solved by technology

However, no one has conducted in-depth research on the extraction and preparation methods of laver polysaccharides so far, but the traditional polysaccharide preparation method has been adopted, which is not targeted, making it difficult to collect laver polysaccharide samples when performing systematic pharmacological screening experiments, resulting in unnecessary manpower , The waste of material resources has caused certain difficulties for further research, development and application in the future. The traditional method of extracting polysaccharides for the extraction of laver polysaccharides mainly has the following shortcomings. There is no certain standard for the temperature and time of the fat-removing method. , it is not easy to grasp, the number of deproteinization methods in organic solvents is more, the amount of organic solvents is large, and the loss of polysaccharides is large. In the method of preparing polysaccharides, ethanol is used to directly precipitate polysaccharides without decolorization treatment, so that the content of polysaccharide pigments is higher. , affecting its use as a drug and health product resource, due to the above reasons, the further expansion of the application range of laver polysaccharides is hindered