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Three-in-one detection reagent plate for early diagnosis of acute myocardial infarction

A technology for acute myocardial infarction and early diagnosis, applied in color/spectral characteristic measurement, measuring devices, instruments, etc., can solve the problems of low diagnostic value of acute myocardial infarction, low sensitivity and low specificity of AMI diagnosis, and achieve cost-effective Low cost, easy operation and simple preparation process

Active Publication Date: 2006-11-22
WUHAN J H BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, commonly used serum enzyme activities (LDH, CPK, GOT, etc.) begin to increase 8 to 10 hours after onset, and have low specificity. They have been gradually eliminated by large hospitals, but grassroots hospitals are still using these indicators due to limited conditions. It is of little value in the diagnosis of early acute myocardial infarction; and the detection of myocardial markers (troponin I, CK-MB, myoglobin) commonly used in large hospitals has a low diagnostic rate for AMI patients within 6 hours of onset , because troponin I and CK-MB did not increase significantly until about 6 hours after the onset of AMI, and the sensitivity to the diagnosis of AMI within 6 hours of onset was low; although myoglobin increased 2 hours after the onset of AMI, it was specific Very poor, higher levels in skeletal muscle than in cardiac muscle

Method used

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  • Three-in-one detection reagent plate for early diagnosis of acute myocardial infarction
  • Three-in-one detection reagent plate for early diagnosis of acute myocardial infarction
  • Three-in-one detection reagent plate for early diagnosis of acute myocardial infarction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The production method of AMI early diagnosis three-in-one detection reagent plate:

[0028] 1. Preparation of monoclonal antibody pairs of GPBB, h-FABP and TpP

[0029] Use purified GPBB, h-FABP and TpP as antigens to immunize BALB / c mice respectively, immunize the second time at an interval of 21 days, and then immunize once at intervals of 10 days for a total of 3-4 times, and then remove the spleen of the immunized mice Cells, the spleen cells were fused with the mouse myeloma cell line F / 0 with PEG, the hybridoma cells were screened on a 96-well cell culture plate using HAT selective medium, and the anti-GPBB, h- The cell lines of FABP and TpP were cloned three times to obtain cell lines stably secreting high-specificity monoclonal antibodies. The ELISA inhibition method was used on the same antigen-coated plate, that is, the cell culture supernatant was added to the reference group, and the cell culture supernatant was added to the inhibition group. The cell cultu...

Embodiment 2

[0041] How to use the three-in-one detection reagent panel for early diagnosis of AMI:

[0042] 1. Detection of whole blood

[0043] Use a dropper to take 100ul of finger blood and drop it into the well of the test plate, and observe the results within 5-15 minutes. Positive when two or more bands appear. Negative when there is only one band. When none of the bands are present, it indicates that the detection board is invalid.

[0044] 2. Detection of serum and plasma

[0045] Use a dropper to take 100ul of centrifuged serum and plasma and drop it vertically into the wells of the detection plate, and observe the results within 5-15 minutes. Positive when two or more bands appear. Negative when there is only one band. When none of the bands are present, it indicates that the detection board is invalid.

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Abstract

The related three-in-one detection agent plate for early diagnosis for AMI comprises: using GPBB, h-FABP and TpP as diagnosis index, fixing colloid gold amrked three antibodies and solid unmarked antibody for one-time detection. This invention is simple, has high sensitivity ans strong specificity, and fit to most place.

Description

technical field [0001] The invention relates to the preparation of antibody pairs of glycogen phosphorylase BB type isozyme (GPBB), heart-type fatty acid binding protein (h-FABP) and thrombosis precursor protein (TpP), and the immunochromatography of colloidal gold labeling and color development Reaction technology and technical field of early diagnosis of acute myocardial infarction (AMI) using antibodies to GPBB, h-FABP and TpP. In particular, it relates to a three-in-one detection reagent board for early diagnosis of acute myocardial infarction. technical background [0002] Acute myocardial infarction (formerly known as acute myocardial infarction, AMI) is a type of coronary heart disease that develops to a severe stage. The main cause of acute myocardial infarction is the sudden blockage of the coronary arteries that supply blood to the heart, resulting in the myocardial infarction supplied by the coronary arteries Degenerative necrosis occurs. 0-3 hours after the ons...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N21/31G01N33/531
Inventor 钱金红廖园园
Owner WUHAN J H BIO TECH
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