Engineered extracellular matrix preparation method
A technology of tissue engineering and extracellular matrix, applied in the field of artificial organs of biomaterials, can solve the problems of poor clinical effect, achieve the effect of repairing malnutrition, easy to use, and preventing recurrence
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Embodiment 1
[0046] Now in conjunction with experiment, preparation method of the present invention is described in further detail:
[0047] 1. Preparation of culture medium
[0048] (1) Basal culture medium
[0049] Ingredient Final Concentration
[0050] Commercial minimum essential medium DMEM 67.5% (v / v)
[0051] Commercial culture medium F12 22.5% (v / v)
[0052] Fetal bovine serum 10% (v / v)
[0053] Insulin 10ng / ml
[0054] Hydrocortisone 30ng / ml
[0055] Adenine 0.22mM
[0056] Transferrin 6mg / ml
[0057] Epidermal Growth Factor 80ng / ml
[0058] Antibiotics 180i.u / ml
[0059] (2) Culture medium 1
[0060] Basal Medium + Bovine Pituitary Extract 0.5% (V / V)
[0061] (3) Culture medium 2
[0062] Basal medium + vitamin C 20μg / ml
[0063] (4) Culture medium 3
[0064] Basal medium + basic fibroblast growth factor 5ng / ml
[0065] 2. Extraction of matrix protein: the present invention adopts the matrix protein mixture extracted from the skin of mammals (can be cattle...
Embodiment 2
[0071] Embodiment 2. burn wound repair test
[0072] 1) Preparation of animal skin deep second-degree scald model: 50kg Yorkie pigs were anesthetized, and the back skin was prepared for disinfection and sterile towels were spread. Using a scald instrument: temperature 100°C, pressure 1kg, time 25s; prepare the burn wound ( Figure 4 ).
[0073] group
Number of cases
Average healing time (days)
Blank control group
10
24.37±3.25
Using the extracellular matrix group of the present invention
10
13.65±3.41
[0074] After using the extracellular matrix repair group prepared by the present invention for 2 weeks, it can be seen that the skin defect has been repaired, the scar is not obvious, and the color is close to the normal skin color ( Figure 5 ).
[0075] Conclusion: The tissue engineered extracellular matrix prepared in the present invention can significantly shorten the healing time of scald wound.
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