Carbon dioxide pressurized sterilization method under normal temperature

Abstract

The present invention discloses normal temperature pressurizing CO2 sterilization method. The sterilization method includes introducing CO2 to eliminate residual air from autoclave, filling liquid to be sterilized to at most 75 vol% of the autoclave, balancing the temperature of the autoclave in water bath at 25-45 deg.c for 10-15 min, introducing CO2 at 2-8 MPa and 25-45 deg.c, and maintaining the pressure for 5-120 min. The present invention has broad spectrum sterilization of killing Gram+ bacterium, Gram- bacterium, saccharomycetes, mildew and other microbes. The normal temperature operation is suitable for sterilizing heat sensitive matter and maintaining food flavor, and lowering pressure while prolonging the pressure maintaining period can reduce the deactivation and hydrolysis of enzyme, protein and other matters. Small amount of alcohol may be added to raise the sterilization effect, save power and reduce operation period.

Description

technical field [0001] The invention relates to a normal temperature and pressurized carbon dioxide sterilization method. Background technique [0002] In the food industry and biotechnology, it is often necessary to sterilize the target system. The most commonly used sterilization method is heat sterilization. The biggest disadvantage of this method is that it is easy to inactivate heat-labile substances such as proteins, amino acids, and enzymes, and also affects the flavor of some foods. Therefore, it is necessary to develop a normal temperature sterilization method. The room temperature sterilization methods that are currently available or are being developed include ultraviolet sterilization, chemical sterilization, ultra-high pressure sterilization, radiation or high-energy electronic sterilization, and oscillating magnetic field sterilization. In 1987, Kamihira et al. found that carbon dioxide under a certain pressure has the effect of killing some microorganisms. Us...

AI Technical Summary

Problems solved by technology

Pressurized carbon dioxide will cause certain damage to the cell membrane, a small amount of intracellular substances leak out, and the cell membrane loses the ability to balance the pH value inside and outside the cell; after the accumulation of carbon dioxide in the cell exceeds the buffer capacity of the cell, the intracellular pH will decrease and the active substances in the cell will be inactivated , the cells cannot perform normal physiological activities and die

[0004] Using pressurized carbon dioxide as a normal temperature sterilization method has not been reported in China.

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