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Insect rhabdovirus insecticide and its prepn. method

A baculovirus and insecticide technology, which is applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of poor dispersibility, inconvenient storage and transportation of suspensions, etc., and achieve a high degree of dispersion, Ease of storage and transportation, and the effect of reducing the amount of use

Inactive Publication Date: 2006-06-14
INST OF ZOOLOGY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the disadvantages of poor dispersibility of existing wettable powders in water and inconvenient storage and transportation of suspensions, so as to provide a product with good dispersibility, easy storage and convenient transportation, and less field consumption. Insect baculovirus insecticide, and preparation method thereof

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Determine the freeze-drying multiple of the virus stock solution: from the Spodoptera litura nuclear polyhedrosis virus stock solution (produced by Jiyuan Baiyun Industrial Co., Ltd. in Henan Province) with a content of 20 billion PIB / grams, extract 100 grams of sample and freeze-dry to obtain a content of 667 grams in 30 grams. 100 million PIB / gram of virus, then the freeze-drying multiple of the original virus drug is 3.3 times.

[0024] Preparation of insect baculovirus insecticide: add 33 grams of sodium dodecylbenzene sulfonate to 1000 grams of the above-mentioned 20 billion PIB / gram of virus stock solution, fully stir, so that sodium dodecylbenzene sulfonate is dissolved in the virus In the moisture between, freeze-dry, obtain 333 grams of 60 billion PIB / grams of Spodoptera litura nucleopolyhedrosis virus insecticide.

[0025] Extract 0.1 gram sample from above-mentioned virus insecticide, add water to 1000mL (dilute 10000 times), stir evenly, count under the micr...

Embodiment 2

[0028] Determine the freeze-drying multiple of the virus stock solution: extract 100 grams of sample from the stock solution of the nuclear polyhedrosis virus of cotton bollworm (Henan Province Jiyuan Baiyun Industrial Co., Ltd. production) whose content is 100 billion PIB / gram obtained from separation and purification, and freeze-dry to obtain 20 grams For a virus with a content of 500 billion PIB / gram, the freeze-drying multiple of the original virus drug is 5 times.

[0029] Preparation of insect baculovirus insecticide: add 2400 grams of sodium dodecylbenzenesulfonate to the virus stock solution of 3000 grams of the above-mentioned 100 billion PIB / gram, fully stir, so that sodium dodecylbenzenesulfonate is dissolved in the virus In the moisture between, freeze-dry, obtain the nuclear polyhedrosis virus insecticide of 3000 grams 100 billion PIB / gram.

[0030] Extract 0.1 gram sample from above-mentioned insect baculovirus insecticide, add water to 1000mL (dilute 10000 times),...

Embodiment 3

[0033] Determine the freeze-drying multiple of the virus stock solution: from the stock solution of the beet armyworm nuclear polyhedrosis virus (produced by Jiyuan Baiyun Industrial Co., Ltd. in Henan Province) with an isolated and purified content of 14 billion PIB / gram, extract 100 grams of sample and freeze-dry to obtain a content of 15.6 grams If it is 90 billion PIB / gram of virus, then the freeze-drying multiple of the original virus drug is 6.43 times.

[0034] Preparation of insect baculovirus insecticide: add 440 grams of sodium lignin to 10,000 grams of the above-mentioned 14 billion PIB / gram of virus, stir well, make the sodium lignin dissolve in the water between the viruses, freeze-dry, and obtain the frozen The dry powder is pulverized to 1000 orders with a jet mill, and 2000 grams of 70 billion PIB / grams of beet armyworm nucleopolyhedrosis virus insecticides are obtained.

[0035] Extract 0.1 gram sample from above-mentioned virus insecticide, add water to 500mL...

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Abstract

An insecticide is proportionally prepared from the baculovirus of insect and surfactant through separating baculovirus from insect, purifying, mixing it with surfactant, freeze drying and pulverizing.

Description

technical field [0001] The invention relates to an insect baculovirus insecticide, in particular to an ultra-low dosage and high content insect baculovirus insecticide preparation and a preparation method thereof. Background technique [0002] Due to the general resistance of agricultural and forestry pests to chemical pesticides, the amount of chemical pesticides per unit area is getting higher and higher, and the harm to the environment, non-target animals, biodiversity and human beings is becoming more and more serious. A large number of highly toxic and high-residue pesticides have been restricted, banned, restricted and banned by the Chinese government. In this context, the production, development and application of insecticides, as the earliest developed and widely used biopesticides, are developing rapidly, and the market demand is increasing year by year. [0003] The use of insect viruses as biological insecticides to control agricultural and forestry pests is an e...

Claims

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Application Information

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IPC IPC(8): A01N63/00
Inventor 秦启联程清泉李瑄苗麟张寰丁翠
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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