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CO2 content determination method and CO2 diagnosis kit

A diagnostic kit and carbon dioxide technology, applied in the field of medical testing and measurement, can solve problems such as lack of experimental research, and achieve the effect of accurate and high-precision test results

Inactive Publication Date: 2006-04-26
王尔中
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The above patents have nothing to do with enzymatic determination, which shows from one aspect that domestic experimental research in this area is very lacking

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] Embodiment one (single agent)

[0112] The carbon dioxide (bicarbonate) diagnostic kit of the present embodiment comprises:

[0113] Buffer 80mmol / l

[0114] Phosphoenolpyruvate 2mmol / l

[0115] Phosphoenolpyruvate Carboxylase 8000U / l

[0116] Inosine 1mmol / l

[0117] Nucleoside phosphatase 6000U / l

[0118] Xanthine oxidase 6000U / l

[0119] Peroxidase 9000U / l

[0120] Urease 6000U / l

[0121] Stabilizer 50% (total volume)

[0122] 4-amino antiarsenic 2mmol / l

[0123] Carbolic acid 10mmol / l

[0124] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 495-505nm, test sub-wavelength above 600nm, the volume ratio of the measured carbon dioxide (bicarbonate) sample to the reagent is 1 / 25 , and the reaction direction is positive.

[0125] After the sample and reagents are added and allowed to mix, the following reactions occur:

[0126] carbon dioxide + phosphoenolpyruvate phosphoenolpyruvate carboxylase

...

Embodiment 2

[0135] Embodiment two (two doses)

[0136] The carbon dioxide (bicarbonate) diagnostic reagent of the present embodiment has:

[0137] Reagent I

[0138] Buffer 100mmol / l

[0139] Phosphoenolpyruvate 6mmol / l

[0140] Phosphoenolpyruvate Carboxylase 10000U / l

[0141] Inosine 3mmol / l

[0142] Nucleoside phosphatase 8000U / l

[0143] Xanthine oxidase 8000U / l

[0144] Peroxidase 15000U / l

[0145] Urease 11000U / l

[0146] Stabilizer 50% (total volume)

[0147] 4-amino antiarsenic 1mmol / l

[0148] Reagent II

[0149] Buffer 100mmol / l

[0150] Phosphoenolpyruvate 6mmol / l

[0151] Phosphoenolpyruvate Carboxylase 10000U / l

[0152] Stabilizer 50% (total volume)

[0153] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 1mmol / l

[0154] When measuring the content of carbon dioxide (bicarbonate), the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 546nm, the secondary wavelength of the test is above 630nm, the volume ratio o...

Embodiment 3

[0165] Embodiment three (three doses)

[0166] The carbon dioxide (bicarbonate) diagnostic reagent of the present embodiment is three doses, has:

[0167] Reagent I

[0168] Buffer 120mmol / l

[0169] Inosine 5mmol / l

[0170] Stabilizer 20mmo1 / l

[0171] 3-methyl-2-benzothiazolone hydrazone 2mmol / l

[0172] Reagent II

[0173] Buffer 120mmol / l

[0174] Nucleoside phosphatase 10000U / l

[0175] Xanthine oxidase 10000U / l

[0176] Peroxidase 20000U / l

[0177]Urease 16000U / l

[0178] Stabilizer 50% (total volume)

[0179] Reagent III

[0180] Buffer 120mmol / l

[0181] Phosphoenolpyruvate 10mmol / l

[0182] Phosphoenolpyruvate Carboxylase 12000U / l

[0183] Stabilizer 50% (total volume)

[0184] Dimethylaniline 2mmol / l

[0185] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 578nm, test sub-wavelength above 700nm, volume ratio of measured carbon dioxide (bicarbonate) sample to reagent is 1 / 25, reaction Directi...

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Abstract

The present invention relates to one CO2 (bicarbonate) content determining method and CO2 (bicarbonate) diagnosis kit, and belongs to the field of medical detection technology. The kit includes buffering solution, phosphoenolpyruvic acid, inosine, phosphoenolpyruvate carboxylase, nucleoside phosphorylase, xanthine oxidase, peroxidase, urease and stabilizer. Through mixing the sample and reagent in certain volume ratio to generate enzyme coupling reaction, detecting the main wavelength absorbance variation of the reaction product under biochemical analyzer and calculation, CO2 (bicarbonate) content is obtained. The present invention has the advantages of high sensitivity, high precision and no contamination of inner and outer matters.

Description

technical field [0001] The invention relates to a method for measuring carbon dioxide (bicarbonate) content, and simultaneously the invention also relates to a carbon dioxide (bicarbonate) diagnostic kit, which belongs to the technical field of medical examination and measurement. Background technique [0002] The content of carbon dioxide in the blood plays an important role in the regulation of acid-base balance in the human body. The constant blood pH is an essential condition for maintaining life activities. Among the various buffer systems in blood plasma, the bicarbonate buffer system is the most important, which directly affects the pH of blood. [0003] Changes in the content of carbon dioxide in the blood mainly reflect metabolic acid-base balance disorders. In pure metabolic acid or alkalosis, blood bicarbonate [HCO 3 - ] decreased or increased, the total carbon dioxide also decreased or increased. In simple respiratory acid or alkalosis, blood bicarbonate rise...

Claims

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Application Information

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IPC IPC(8): C12Q1/42
Inventor 王尔中
Owner 王尔中
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