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Preparation method of dried active amnion

An amniotic membrane and active technology, which is applied in the field of preparation of decellularized and dried active amniotic membrane, can solve the problems of difficult to take and store at any time, difficulty in clinical application, etc., and achieve the effect of convenient clipping, convenient storage, and reduced antigenicity

Active Publication Date: 2006-04-12
HE EYE HOSPITAL SHENYANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the amniotic membranes used clinically are fresh amniotic membranes or cryopreserved amniotic membranes, which are difficult to take and store at any time.
Although the biological amniotic membrane product with the patent publication number CN1522568A is a freeze-dried amniotic membrane that can be stored at room temperature, it does not remove the cells of the amniotic membrane, which brings difficulties to clinical application, and cannot be used as tissue engineering materials for epithelial and endothelial culture and transplantation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The placenta tissue of healthy cesarean section women was selected, and prenatal serological examination was performed to exclude hepatitis B virus (HBV), hepatitis C virus (HCV), cytomegalovirus, Treponema pallidum and immunodeficiency virus (HIV) infection in accordance with relevant national standards. After the placenta is obtained from cesarean section or aseptic delivery by vaginal delivery, it is operated under aseptic conditions and sterilized as follows: rinse the blood on the surface of the placenta with normal saline; soak the placenta with sterile normal saline containing antibiotics for 5 to 10 minutes (antibiotics mixed The concentration of the solution is 50ug / ml penicillin, 50ug / ml streptomycin, 100ug / ml neomycin and 2.5ug / ml amphotericin B).

[0013] Separate the amnion from the chorion, scrape off the sponge layer as much as possible with the side of the glass slide, spread the epithelial side up and fix it on nitrocellulose paper (provided by Bio-Tissu...

Embodiment 2

[0015] The placenta tissue of healthy cesarean section women was selected, and prenatal serological examination was performed to exclude hepatitis B virus (HBV), hepatitis C virus (HCV), cytomegalovirus, Treponema pallidum and immunodeficiency virus (HIV) infection in accordance with relevant national standards. After the placenta is obtained from cesarean section or aseptic delivery by vaginal delivery, it is operated under aseptic conditions and sterilized as follows: rinse the blood on the surface of the placenta with normal saline; soak the placenta with sterile normal saline containing antibiotics for 5 to 10 minutes (antibiotics mixed The concentration of the solution is 50ug / ml penicillin, 50ug / ml streptomycin, 100ug / ml neomycin and 2.5ug / ml amphotericin B).

[0016] Separate the amnion from the chorion, scrape off the sponge layer as much as possible with the side of the glass slide, spread the epithelial side up and fix it on nitrocellulose paper (provided by Bio-Tissu...

Embodiment 3

[0018] The placenta tissue of healthy cesarean section women was selected, and prenatal serological examination was performed to exclude hepatitis B virus (HBV), hepatitis C virus (HCV), cytomegalovirus, Treponema pallidum and immunodeficiency virus (HIV) infection in accordance with relevant national standards. After the placenta is obtained from cesarean section or aseptic delivery by vaginal delivery, it is operated under aseptic conditions and sterilized as follows: rinse the blood on the surface of the placenta with normal saline; soak the placenta with sterile normal saline containing antibiotics for 5 to 10 minutes (antibiotics mixed The concentration of the solution is 50ug / ml penicillin, 50ug / ml streptomycin, 100ug / ml neomycin and 2.5ug / ml amphotericin B).

[0019] Separate the amnion from the chorion, scrape off the sponge layer as much as possible with the side of the glass slide, spread the epithelial side up and fix it on nitrocellulose paper (provided by Bio-Tissu...

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Abstract

A process for preparing the dried active amnion includes such steps as choosing health placenta tissue, sterilizing, separating amnion, removing sponge layer, spreading it on nitro-cellular paper, shearing by needed sizes, incubating in trypsase solution and / or EDTA solution, removing epithelial cells, flushing by physiologic saline, vacuum freeze drying, vacuum packing and gamma-ray radiating.

Description

Technical field [0001] The invention relates to medical amniotic membrane products, in particular to a method for preparing decellularized and dried active amniotic membrane which can be stored for a long time. Background technique [0002] The amniotic membrane, which evolved from the cytotrophoblast, is the inner layer of the human two-layer fetal membrane and is the thickest basement membrane in the human body. Amniotic membrane itself has no blood vessels, nerves, and lymphatic vessels, and its antigenicity is low (mainly due to its cellular components). The application of amniotic membrane first began in 1910, and Divis first used fetal membrane (amniotic membrane + chorion) for transplantation and achieved success. Later, amniotic membrane was widely used in various operations as burn skin grafts and artificial vaginal mucosal sheaths. Until 1995, when Kim and Tseng performed amniotic membrane transplantation on the surface of the cornea in a chemical burn model, amni...

Claims

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Application Information

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IPC IPC(8): C12N5/08A01N1/02C12N5/073
Inventor 何伟
Owner HE EYE HOSPITAL SHENYANG
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