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Bi-recombinant active carrier vaccine of co-expression pig gyrate virus and foot and mouth disease virus

A technology of porcine circovirus and foot-and-mouth disease virus, applied in the direction of viral peptides, antibody medical ingredients, medical preparations containing active ingredients, etc., can solve the problems of no effective treatment methods and no effective vaccines available

Inactive Publication Date: 2006-03-22
金宁一
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] There is no effective treatment for this disease, and no effective vaccine is available at home and abroad

Method used

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  • Bi-recombinant active carrier vaccine of co-expression pig gyrate virus and foot and mouth disease virus
  • Bi-recombinant active carrier vaccine of co-expression pig gyrate virus and foot and mouth disease virus
  • Bi-recombinant active carrier vaccine of co-expression pig gyrate virus and foot and mouth disease virus

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Embodiment Construction

[0182] 1. Preparation and transformation of E. coli competent cells, plasmid extraction and restriction enzyme digestion, DNA fragment recovery, linear DNA fragment connection, recombinant plasmid screening and identification, PCR amplification reaction, etc. Refer to Jin Dongyan and Li Mengfeng Translated "Molecular Cloning Experiment Guide" second edition, Science Press (1992) related chapters.

[0183] 1.1 Preparation of E. coli competent cells

[0184] Under aseptic conditions, pick the strains stored in the refrigerator at -70°C, streak them and inoculate them on LB agar plates without ampicillin (Amp), and invert them at 37°C for about 16 hours. Pick a single colony, inoculate it into 3ml of LB culture medium without antibiotics, and cultivate overnight at 37°C with shaking. The next day, 1ml of bacterial culture was aseptically drawn and added to 100ml of Amp-free LB culture that was preheated to 37°C, and cultured at 37°C with vigorous shaking (200r / min). Cultivate to OD 6...

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Abstract

The present invention belongs to the field of biotechnology, and is especially one kind of recombinant live carrier vaccine co-expressing pig gyrate virus and foot and mouth disease virus. The present invention aims at providing two recombinant chicken pox viruses to co-express capsid protein ORF2 or its fusion protein ORF2-ORF1 of the China epidemic strain of type-II pig gyrate virus and the structural protein precursor P1 of the China epidemic strain NY00 of type-O foot and mouth disease virus, and for serving as the duplex live carrier gene engineering vaccine to prevent PCV2 and FMDV infection. The two recombinant chicken pox viruses can stimulate mouse to generate effective body fluid immunity and cell immunity, and are safe to experimental animals without any pathological phenomenon.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and particularly relates to a recombinant live vector vaccine co-expressing porcine circovirus and foot-and-mouth disease virus. Background technique: [0002] Porcine Circovirus (PCV) is the smallest animal virus found so far. Porcine circovirus type 2 (PCV2) is pathogenic and can cause multiple system failure syndrome (PMWS) in weaned piglets. It is a new infectious disease. The first outbreak of the disease in Canada in 1991, followed by reports of the disease in many countries and regions in the world, causing serious economic losses to the pig industry. There are also reports of PMWS in their pig herds in Taiwan, Jiangsu and Hebei in the mainland of China. PMWS not only brings considerable economic losses to pig production, but also poses a potential threat to public health. Especially when it comes to xenotransplantation, it is necessary to assess the risk of new non-characteristic pig pathogens. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/42C12N15/863C07H21/00C07K14/09A61K39/295A61K39/135A61K48/00
Inventor 金宁一秦晓冰郑敏尹革芬金扩世夏志平李昌费东亮金洪涛
Owner 金宁一
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