Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sperm nucleoprotein mode converting semi-quantitative detection reagent and detection thereof

A semi-quantitative detection and nucleoprotein technology, applied in the field of semi-quantitative detection of sperm nucleoprotein histotype conversion, can solve the problems of high requirements for personnel and equipment, unsuitable clinical routine development, unreasonable reagent formulations, etc., and achieves standardized methodology. , to achieve batch detection, consistent dyeing effect

Inactive Publication Date: 2006-03-15
深圳华康生物医学工程有限公司
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former method is complicated to operate and has relatively high requirements for personnel and equipment, so it is not suitable for routine clinical practice; the latter method is simple to operate, but due to the unreasonable reagent formula, it causes large background noise and low specificity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] Embodiment, a semi-quantitative detection reagent for sperm nucleoprotein karyotype conversion, including staining solution, washing solution, binding solution, fixative solution, eluent and mounting solution; because the detection reagent proposed by the present invention detects immature The number of sperm, and the reagent of usual quantitative detection sperm nucleoprotein karyotype conversion should be to detect the absolute amount of the histone that contains lysine residue, therefore, detection reagent of the present invention is sperm nucleoprotein karyotype conversion semi-quantitative The reagent of detection; Wherein: washing liquid is used for washing away the viscous part of sperm surface, and described washing liquid adopts the phosphate (PBN) buffer solution of pH7.4 in the present invention, and described washing liquid is that 1000ml distilled water contains phosphoric acid Potassium dihydrogen (KH 2 PO 4 ) 0.5g, sodium hydrogen phosphate dodecahydrate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A semi quantitative detection method of constitutive conversion for sperm nucleoprotein includes adding the binding solution in semen and coating it on staining window of glass slide to make sperm stick on glass slide easily, adding the staining solution in said semen to stain semen, mounting semen and counting out percentage of sperm with blue head from certain amount of sperms. The reagent for realizing the method consists of staining solution, binding solution for sticking semen on glass slide easily and eluent for washing out nonspecific staining after semen being stained.

Description

【Technical field】 [0001] The invention belongs to the category of in vitro diagnostic reagents, and is particularly suitable for the semi-quantitative detection of sperm nucleoprotein group conversion. 【Background technique】 [0002] The nuclear protein type of human spermatogonia is mainly histone rich in lysine residues. During the process of sperm maturation, the basic protein combined with sperm nuclear DNA undergoes a type conversion, and the histone rich in lysine residues gradually Replaced by protamine, which is rich in arginine and cystine residues, the nucleoprotein type of mature sperm is mainly protamine. [0003] The defect or abnormality of proteotype conversion can cause male infertility or early embryonic death and miscarriage. Unable to depolymerize normally, which affects the fusion of male and female pronuclei; ③The embryo cannot develop normally, resulting in premature embryo death and miscarriage. [0004] There are two methods for detection of sperm n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/25G01N15/10G01N1/30G01N1/28
Inventor 傅剑华刘瑜胡家纯何林何小红
Owner 深圳华康生物医学工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com