Use of high wavenumber raman spectroscopy for measuring tissue

A technology of organization and purpose, applied in optical radiation measurement, radiation pyrometry, spectrometry/spectrophotometry/monochromator, etc., can solve problems such as unsatisfactory signal-to-noise ratio

Inactive Publication Date: 2006-03-08
RIVER DIAGNOSTICS
View PDF3 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This use also results in an unsatisfactory signal-to-noise ratio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of high wavenumber raman spectroscopy for measuring tissue
  • Use of high wavenumber raman spectroscopy for measuring tissue
  • Use of high wavenumber raman spectroscopy for measuring tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] Example 1: Raman mapping of atherosclerotic arteries

[0111] This experiment describes the possibility of Raman spectroscopy in the spectral region of the present invention for studying atherosclerotic plaques.

[0112] The human coronary artery samples used to generate the Raman maps shown in Figure 1 were obtained at autopsy (less than 24 hours postmortem). They were snap frozen in liquid nitrogen and stored at -80°C until use. For Raman measurements, a 20 μm thick cryosection was placed in calcium fluoride (CaF 2 ) window (Crystran UK) and passively heated to room temperature. Raman measurements were followed by staining using standard hematoxylin and eosin staining procedures.

[0113] To collect Raman spectra, a 719 nm laser from an argon-ion pumped titanium:sapphire laser system (Spectra Physics, Mountain View, CA) was used. The Raman microspectrometer system used is described in detail in Van de Poll SWE, Bakker Schut TC, Van der Laarse A, Puppels GJ "In Sit...

Embodiment 2

[0130] Example 2: Raman mapping of cancer tissue

[0131] This experiment describes the possibility of Raman spectroscopy in the spectral region of the invention for studying cancerous tissue.

[0132] High wavenumber regions can also be beneficially used in various clinical applications. For example, Figure 4A shows a Raman map obtained from a thin tissue section of meningioma-infiltrated human dura in a manner similar to the map of an atherosclerotic lesion of Figure 1A. It is not currently possible to evaluate the cut margins well intraoperatively. However, it is known that remaining meningioma tissue may lead to tumor recurrence. Figure 4B shows pictures of adjacent tissue sections after staining with hematoxylin and eosin (H&E staining). Surprisingly, histopathological evaluation of this section and its comparison with the Raman map showed that the light gray areas of the Raman map corresponded to the dura, while the dark gray areas corresponded to meningiomas (MGs). ...

Embodiment 3

[0134] Example 3: Raman Mapping of Cancer Tissue

[0135] This experiment describes the possibility of Raman spectroscopy in the spectral region of the present invention for studying cancerous tissue.

[0136] Figure 5A shows a Raman map of a thin section of a human glioblastoma with both major tumor areas and areas of necrotic tissue. Surprisingly, comparison of the Raman map, evaluated by a neuropathologist, with the H&E-stained adjacent section revealed light gray areas corresponding to primary tumor tissue, whereas dark gray areas in the Raman map corresponded to necrotic tissue.

[0137] This experiment shows that the Raman determination of the spectral region of the present invention provides valuable information on cancerous tissue of the brain, enabling Raman spectroscopy to distinguish primary tumor tissue from necrotic tissue.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention is related to the instrument for measuring a Raman signal of tissue, the instrument comprising a laser, a signal detection unit for measuring the Raman signal, and a fiber optic probe, wherein the fiber optic probe comprises one or more optical fibers for directing laser light onto the tissue and for collecting light that is scattered by the tissue and guiding the collected light away from the tissue towards the signal detection unit, wherein the fiber or fibers for collecting light have substantially no Raman signal in one or more parts of the 2500-3700 cm<-1 >spectral region, and wherein the detection unit records the Raman signal scattered by the tissue in said spectral region. The invention enables ex vivo, in vitro and in vivo analysis and diagnosis of atherosclerotic plaque and detection of tumor tissue with great advantages over current state-of-the-art technology.

Description

technical field [0001] The invention relates to an instrument for measuring Raman signal of tissue and its application, the instrument comprises a laser, a signal detection device and an optical fiber probe. Background of the invention [0002] Atherosclerosis is an important cause of death in many parts of the world. Therefore, many techniques have been developed to obtain information on plaques in blood vessels. Imaging techniques such as angiography, magnetic resonance imaging, intravascular ultrasound and optical adhesion tomography provide information on the location of plaque or vessel blockage as well as the morphology or internal structure of the plaque. However, they cannot analyze the molecular composition of plaques in detail in vivo. Information on the molecular composition of plaques is important for determining the probability of acute cardiac events. A distinction is made between so-called stable plaques and vulnerable plaques, which are thought to be respo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01J3/44A61B5/00G01N21/35G01N21/359
CPCG01J3/44A61B5/4848A61B5/441A61B5/0071G01N21/65G01N2021/656A61B5/0086A61B5/0075
Inventor 格温·扬·普珀尔斯拉尔夫·沃尔休伊斯塞纳达·科尔叶诺维克
Owner RIVER DIAGNOSTICS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap