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Bilobalide extraction and purification process

A ginkgolide and process technology, applied in the field of traditional Chinese medicine pharmacy, can solve the problems that scientific research, production and application cannot be satisfied, the cost of ginkgolide process is high, the content of ginkgolide is low, etc., and the cost is low, the price is low, and the operation is simple. Effect

Active Publication Date: 2006-02-15
SICHUAN ENWEI TCM RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of ginkgolides in ginkgo leaves is low, and the current process for extracting ginkgolides is high in cost and low in yield, which cannot meet the needs of scientific research, production and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 200 grams of ginkgo leaves and pulverize them, add 80% ethanol to reflux and extract 3 times, the amount of ethanol each time is 8 times of the weight of the medicinal material, and the reflux time is 2 hours each time. Combine the extracts for 3 times, recover ethanol until there is no alcohol smell, degrease with gasoline 5 times, extract the water phase with butanone 5 times, combine the butanone solution, wash with saturated lime water and remove impurities, recover the butanone solution, and obtain Ginkgo biloba The crude ester was recrystallized with 40% ethanol solution, allowed to stand, filtered, and dried at 90°C to obtain the refined product of ginkgolide.

[0026] It has been determined that the transfer rate of ginkgolides is over 60%, the total content of ginkgolides A, B, C and bilobalide is over 92%, of which the content of ginkgolide B is over 40%, and the main impurity, ginkgolic acid, is less than 100%. 2 out of 10,000.

Embodiment 2

[0028] Weigh 200 grams of ginkgo leaves and pulverize them, add 70% ethanol to reflux and extract 3 times, the amount of ethanol each time is 7 times the weight of the medicinal material, and the reflux time is 2.5, 2.5, 2 hours respectively. Combine the extracts for 3 times, recover ethanol until there is no alcohol smell, degrease with carbon tetrachloride for 5 times, extract the water phase with ether for 6 times, combine the ether solution, wash with 0.4% borax aqueous solution and remove impurities, recover ether, and obtain ginkgolide The crude product was recrystallized with 50% ethanol solution, allowed to stand, filtered, and dried at 90°C to obtain the refined product of ginkgolide.

[0029] It has been determined that the transfer rate of ginkgolides is over 60%, the total content of ginkgolides A, B, C and bilobalide is over 92%, of which the content of ginkgolide B is over 40%, and the main impurity, ginkgolic acid, is less than 100%. 2 out of 10,000.

Embodiment 3

[0031] Weigh 200 grams of Ginkgo biloba leaves and pulverize them, add 10% methanol for warm soaking and extracting 3 times, the amount of methanol each time is 10 times of the weight of the medicinal material, and warm soaking time is 5 hours each time. Combine the extracts for 3 times, recover methanol, degrease with carbon tetrachloride 5 times, extract the water phase with ether 6 times, combine the ether solution, wash with 0.5% potassium bicarbonate solution to remove impurities, recover ether, and obtain crude ginkgolide , recrystallized with 70% ethanol solution, allowed to stand, filtered, and dried at 80°C to obtain refined ginkgolide.

[0032] It has been determined that the transfer rate of ginkgolides is over 60%, the total content of ginkgolides A, B, C and bilobalide is over 92%, of which the content of ginkgolide B is over 40%, and the main impurity, ginkgolic acid, is less than 100%. 2 out of 10,000.

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PUM

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Abstract

The invention discloses a process for extracting and purifying gingko total terpene lactone, which comprises, weighing dried leaves of ginkgo, disintegrating, extracting with or ethanol or methanol, reclaiming alcohols from the extract, removing impurities with defatted solvents, extracting and removing impurities again, obtaining the crude product, recrystallizing with ethanol, finally filtering and drying.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a process for extracting and purifying ginkgolide, the main active component of ginkgo leaves, from ginkgo leaves. Background technique [0002] Ginkgolides are highly active platelet activating factor antagonists that are unanimously recognized in literature at home and abroad. Platelet activating factor is an important mediator in the pathogenesis of cardiovascular and cerebrovascular diseases. In 2001, Zhang Yuyang from Shenyang Pharmaceutical University proved that ginkgolide B is a strong inhibitor of platelet activating factor, while ginkgolides A and C have synergistic effects. Ginkgolide 32mg / kg can reduce the death time of mice; 4, 8, 16mg / kg can reduce the scope of cerebral infarction in MCAO rats, improve behavioral disorders and pathological morphology of brain tissue. In the same year, Wang Qiujuan of China Pharmaceutical University proved that gi...

Claims

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Application Information

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IPC IPC(8): C07D493/22C07D307/00
Inventor 曹庆先楼凤昌成仁曼薛永新薛永宏时丹
Owner SICHUAN ENWEI TCM RES & DEV
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