Alzheimer's disease model mouse latent infected by human cytomegalovirus, and its establishing mehtod
A technology of human cytomegalovirus and latent infection, applied in the direction of pharmaceutical formulations, in vivo test preparations, etc., can solve the problem of not revealing the essence of AD development, lack of ideal animal models, and inability to fully reflect the pathological changes of AD, etc. question
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[0023] 1. Establishment and identification of HCMV congenital latent infection mouse model:
[0024] The HCMV AD169 strain was selected to recover and culture on human fibroblasts (prepared by the present invention), and virus titration was carried out. When the virus reached 1×105 PFU, 1 ml of each mouse was used as the inoculation volume, and a DMEM control group was set up. After intraperitoneal inoculation of 8-week-old Balb / c SPF male and female mice (raised in a shielding system), they were paired according to female and male 1:1, and divided into virus-infected group and DMEM control group. One year after the progeny mice, 20 progeny mice of the virus group were randomly selected, and the progeny mice of the virus group were divided into an inactivated group and an activated group with 10 each, and 20 mice in the control group were also divided into the inactivated group and the activated group There were 10 rats in each active group. Do the following experiments:
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example 2
[0038] 1. Establish HCMV latent infection mouse model and identify the model:
[0039] Infect 8-week-old Balb / c SPF male and female mice with 1×105 PFU of the HCMV AD169 strain, and set up a DMEM control group. After feeding for one year, randomly select 20 mice from the virus-infected group and 20 corresponding control mice to determine The infection state of the mice in the virus infection group was latent infection;
[0040] 2. Identification of Alzheimer's disease model mice induced by HCMV latent infection:
[0041] After confirming the infection status of the mice, 10 mice from the virus infection group and 10 mice from the control group were randomly selected for behavioral experiments on mice. The mice were preliminarily determined to be senile dementia mice. After the behavioral experiments, the mice were killed and carried out. The neuropathological examination of the brain tissue confirmed the neurofibrillary tangles (neurofibrillary tangles, NFTs) in the nerve cel...
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