Alzheimer's disease model mouse latent infected by human cytomegalovirus, and its establishing mehtod

A technology of human cytomegalovirus and latent infection, applied in the direction of pharmaceutical formulations, in vivo test preparations, etc., can solve the problem of not revealing the essence of AD development, lack of ideal animal models, and inability to fully reflect the pathological changes of AD, etc. question

Inactive Publication Date: 2006-01-25
王明丽
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Problems solved by technology

[0004] One of the difficulties in the prevention and treatment of AD is the lack of corresponding ideal animal models
An ideal model should reproduce the behavioral abnormalities, neuropathological features, and neurobiochemical changes of AD. Although many AD models have been used in existing experiments, they are all based on different theories, and most of them use single factor modeling. Can not fully reflect the pathological changes of AD, such as cholinergic damage model, abnormal metabolism model, natural aging model, aluminum poisoning model
At present, the ideal models for studying aging and dementia are the rapid aging mouse subline 8 (SAMP8) and transgenic models. However, these models are not only expensive and difficult to popularize, but also cannot reveal the nature of the development of AD well. An animal model that can more comprehensively simulate the neuropathology of AD disease has not been reported yet

Method used

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example 1

[0023] 1. Establishment and identification of HCMV congenital latent infection mouse model:

[0024] The HCMV AD169 strain was selected to recover and culture on human fibroblasts (prepared by the present invention), and virus titration was carried out. When the virus reached 1×105 PFU, 1 ml of each mouse was used as the inoculation volume, and a DMEM control group was set up. After intraperitoneal inoculation of 8-week-old Balb / c SPF male and female mice (raised in a shielding system), they were paired according to female and male 1:1, and divided into virus-infected group and DMEM control group. One year after the progeny mice, 20 progeny mice of the virus group were randomly selected, and the progeny mice of the virus group were divided into an inactivated group and an activated group with 10 each, and 20 mice in the control group were also divided into the inactivated group and the activated group There were 10 rats in each active group. Do the following experiments:

[...

example 2

[0038] 1. Establish HCMV latent infection mouse model and identify the model:

[0039] Infect 8-week-old Balb / c SPF male and female mice with 1×105 PFU of the HCMV AD169 strain, and set up a DMEM control group. After feeding for one year, randomly select 20 mice from the virus-infected group and 20 corresponding control mice to determine The infection state of the mice in the virus infection group was latent infection;

[0040] 2. Identification of Alzheimer's disease model mice induced by HCMV latent infection:

[0041] After confirming the infection status of the mice, 10 mice from the virus infection group and 10 mice from the control group were randomly selected for behavioral experiments on mice. The mice were preliminarily determined to be senile dementia mice. After the behavioral experiments, the mice were killed and carried out. The neuropathological examination of the brain tissue confirmed the neurofibrillary tangles (neurofibrillary tangles, NFTs) in the nerve cel...

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Abstract

A model mouse with Alzheimer's disease (AD) caused by the dormant infection of human cytomegalovirus for researching the cause and pathogenesis of AD to provide a tool for preventing and treating AD is disclosed. Its creating method features that the cytomegalovirus strain HCMV AD169 is used to infect the Balb / c SPF-class male and female mice with 8-week age to create the filial mouse model.

Description

technical field [0001] The invention relates to a Balb / c model mouse of Alzheimer's disease (AD) caused by human cytomegalovirus (HCMV) latent infection, which is mainly used for studying the etiology, pathology, pathogenesis, prevention and treatment of human AD disease. Background technique [0002] AD (A1zheimer's disease) is a disease of progressive central nervous system decline, also known as senile dementia. In the elderly, the incidence rate of AD is very high. The incidence rate of AD in the population over 65 years old in the world is 5% to 10%. In the United States, AD disease has become the fourth leading cause of death. With the average life expectancy of our population The prolongation of the aging population, the increasing number of AD patients, and the two epidemiological surveys in 1989 and 1990 showed that the prevalence of AD in the population over 60 years old in my country was 3.46%-6.41%, and it was on the rise. The disease has become It is a serious di...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 王明丽
Owner 王明丽
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