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Alzheimer's disease model mouse latent infected by human cytomegalovirus, and its establishing mehtod

A technology of human cytomegalovirus and latent infection, which is applied in the direction of pharmaceutical formulations and preparations for in vivo experiments, etc., can solve the problems of high price, lack of ideal animal models, and inability to fully reflect the pathological changes of AD

Inactive Publication Date: 2007-09-05
王明丽
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  • Description
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AI Technical Summary

Problems solved by technology

[0004] One of the difficulties in the prevention and treatment of AD is the lack of corresponding ideal animal models
An ideal model should reproduce the behavioral abnormalities, neuropathological features, and neurobiochemical changes of AD. Although many AD models have been used in existing experiments, they are all based on different theories, and most of them use single factor modeling. Can not fully reflect the pathological changes of AD, such as cholinergic damage model, abnormal metabolism model, natural aging model, aluminum poisoning model
At present, the ideal models for studying aging and dementia are the rapid aging mouse subline 8 (SAMP8) and transgenic models. However, these models are not only expensive and difficult to popularize, but also cannot reveal the nature of the development of AD well. An animal model that can more comprehensively simulate the neuropathology of AD disease has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] 1. Establishment and identification of HCMV congenital latent infection mouse model:

[0024] Select the HCMV AD169 strain to resuscitate and cultivate on human fibroblasts (prepared by the present invention), and carry out virus titration until the virus reaches 1 × 10 5 For PFU, 1 ml per mouse was used as the inoculation volume, and a DMEM control group was established. After intraperitoneal inoculation of 8-week-old Balb / c SPF male and female mice (raised in a shielding system), they were paired according to female and male 1:1, and divided into virus-infected group and DMEM control group. One year after the progeny mice, 20 progeny mice of the virus group were randomly selected, and the progeny mice of the virus group were divided into an inactivated group and an activated group with 10 each, and 20 mice in the control group were also divided into the inactivated group and the activated group There were 10 rats in each active group. Do the following experiments: ...

example 2

[0038] 1. Establish HCMV latent infection mouse model and identify the model:

[0039] Take 1×10 5 The HCMV AD169 strain of PFU was used to infect 8-week-old Balb / c SPF male and female mice, and a DMEM control group was set up. After feeding for one year, 20 mice in the virus-infected group and 20 control mice were randomly selected to determine the size of the virus-infected group. The infection status of mice is latent infection;

[0040] 2. Identification of Alzheimer's disease model mice caused by HCMV latent infection:

[0041] After confirming the infection status of the mice, 10 mice from the virus infection group and 10 mice from the control group were randomly selected for behavioral experiments on mice. The mice were preliminarily determined to be senile dementia mice. After the behavioral experiments, the mice were killed and carried out. The neuropathological examination of the brain tissue confirmed the neurofibrillary tangles (neurofibrillary tangles, NFTs) in ...

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Abstract

A model mouse with Alzheimer's disease (AD) caused by the dormant infection of human cytomegalovirus for researching the cause and pathogenesis of AD to provide a tool for preventing and treating AD is disclosed. Its creating method features that the cytomegalovirus strain HCMV AD169 is used to infect the Balb / c SPF-class male and female mice with 8-week age to create the filial mouse model.

Description

technical field [0001] The invention relates to a Balb / c model mouse of Alzheimer's disease (AD) caused by human cytomegalovirus (HCMV) latent infection, which is mainly used for studying the etiology, pathology, pathogenesis, prevention and treatment of human AD disease. Background technique [0002] AD (Alzheimer's disease) is a disease of progressive central nervous system decline, also known as senile dementia. In the elderly, the incidence rate of AD is very high. The incidence rate of AD in the population over 65 years old in the world is 5% to 10%. In the United States, AD disease has become the fourth leading cause of death. With the average life expectancy of our population The prolongation of the aging population, the increasing number of AD patients, and the two epidemiological surveys in 1989 and 1990 showed that the prevalence of AD in the population over 60 years old in my country was 3.46%-6.41%, and it was on the rise. The disease has become It is a serious di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 王明丽
Owner 王明丽
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