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Avian influenza immunogold diagnosis test strip and test card

A colloidal gold and test card technology, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of different sources of raw materials, reagent preparation and production processes, and high detection prices, and achieve the effects of low price and sensitivity and specificity.

Inactive Publication Date: 2005-11-23
重庆工学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Faster and more accurate techniques are ELISA and fluorescent RT-PCR, but these two techniques require special equipment, and the detection time is more than 2 hours, and the detection price is relatively expensive
All of these technical methods cannot be used in grass-roots production sites, quarantine stations, and clinical sites, and special laboratories must be established; although immunocolloidal gold chromatography is a relatively mature technology, it is not suitable for the application of different antigen-antibody systems. There are differences, including raw material sources, reagent preparations, production processes, etc., requiring new designs and experiments
Therefore, there are no test strips and test cards for the rapid diagnosis of avian influenza immune colloidal gold chromatography in China, including literature reports and invention patents.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Dilute the inactivated avian influenza virus standard antigen with normal non-immune chick serum by 1: 10-1: 10000, detect it with the assembled AIV immune colloidal gold test strip, and it is positive and can be detected The maximum dilution is 1:8000, and the HA test can only detect 1:100. At the same time, chicken Newcastle disease virus (NDV) weak point vaccine was diluted with chick serum, and the test strips were detected to contain NDV-containing chicken serum and normal chicken serum, both of which were negative. It shows that the AIV immune colloidal gold reagent strip has good specificity and sensitivity.

Embodiment 2

[0021] Example 2 Dilute the inactivated avian influenza virus antigen with normal non-immune chick serum by 1: 10-1: 10000, detect it with the assembled AIV immune colloidal gold test card, and if it is positive, the maximum dilution that can be detected The degree is 1:8000, and the HA test can only detect 1:100. At the same time, chicken Newcastle disease virus (NDV) weak point vaccine was diluted with chick serum, and the test card detected chicken serum containing NDV and normal chicken serum, both of which were negative. It shows that the AIV immune colloidal gold test card has good specificity and sensitivity.

Embodiment 3

[0022] Example 3 After the AIV inactivated antigen is purified, the protein concentration is measured with a UV spectrophotometer, and then diluted with normal non-immune chick serum 10 times to 100ug / ml, 10ug / ml, 1ug / ml, 100ng / ml, 10ng / ml, 1ng / ml, 0.1ng / ml, and make the same dilution of NDV at the same time. Use AIV immune colloidal gold reagent strip and test card to detect respectively. Results The reagent strip and test card could detect AIV at a concentration of 10ng / ml, while NDV and normal serum were all negative. Thereby demonstrating the sensitivity and specificity of the diagnostic reagent strips and test cards.

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PUM

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Abstract

The invention relates to a new technology for measuring AIV called colloid gold immune color printing technology, which uses this technology to make test paper and test card for diagnosing AIV rapidly. The invention develops AIV monoclonal antibody: Antibody 1, sheep resistant AIV polyclonal antibody: Antibody 2 and rabbit resistant Balb / C small mouse immune protein antibody: Antibody 3. Ab1 labels colloid gold as gold antibody, Ab2 as capture antibody and Ab3 as quality controlled antibody. It uses antibody, pyroxylin film (NC film), covering pad, sample pad, backing, and plastic card and so on to assembly process test paper and test card. The test paper and test card can test AIV in the ill animal's blood and stool. It uses 10 minutes with the sensibility of 10ng / ml.

Description

a technical field [0001] Avian influenza immune colloidal gold chromatography test strips and test cards belong to a new kind of animal diagnostic reagents, which are mainly used to diagnose animals infected by avian influenza virus, and secondly, they can also detect whether humans are infected with avian influenza virus. The test strip uses the principle of immunological antigen-antibody specific binding, and the colloidal gold-labeled antibody (AIV monoclonal antibody) is combined with the antigen (AIV), and the antigen-antibody conjugate is combined with the AIV antibody to form a sandwich antigen-antibody combination The color reaction of the substance on the NC film can be observed with the naked eye. Therefore, this technology belongs to the field of immunological diagnosis technology, but its technology is mainly used in the field of animal husbandry and veterinary medicine and the field of human medicine. The products are mainly used in the field of animal quarantine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
Inventor 蔡家利姜和吴胜昔王健
Owner 重庆工学院
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