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Prepn. and application of nanometer molecule prepn. with fused immunity pig gene used for anti-infection

A technology that fuses genes and nano-molecules, used in anti-infective drugs, gene therapy, drug combinations, etc.

Inactive Publication Date: 2005-11-09
SICHUAN JINZHAO BIOTECH
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  • Abstract
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AI Technical Summary

Problems solved by technology

At present, there is no report on the research on porcine IL-4 and IL-6 gene recombination and fusion as a molecular immune enhancer at home and abroad

Method used

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  • Prepn. and application of nanometer molecule prepn. with fused immunity pig gene used for anti-infection
  • Prepn. and application of nanometer molecule prepn. with fused immunity pig gene used for anti-infection
  • Prepn. and application of nanometer molecule prepn. with fused immunity pig gene used for anti-infection

Examples

Experimental program
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Effect test

Embodiment Construction

[0090] 1. Cloning and fusion of porcine IL4, 6 genes (PIL-4, 6 genes)

[0091] (1) Cloning of porcine IL4 and IL6 genes

[0092] Cloning of Interleukin-4 Gene of Tibetan Pig

[0093] The peripheral blood lymphocytes of Tibetan pigs were separated by lymphocyte separation medium, cultured in vitro for 36 hours under the stimulation of Con A, the total RNA of the cultured lymphocytes was extracted, and the expression of interleukin-4 gene of Tibetan pig lymphocytes was amplified by RT-PCR. The cDNA was cloned into the pMD18-T vector, and the sequence analysis was carried out; the homologous comparison of the sequence obtained in the GenBank and EMBL databases confirmed that the cDNA of Tibetan pig interleukin-4 was cloned in this experiment.

[0094] Cloning of porcine interleukin-6 gene

[0095] The peripheral blood lymphocytes of Chenghua pigs were separated by lymphocyte separation medium, cultured in vitro for 24 hours under the stimulation of ConA, the total RNA of activa...

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Abstract

An anti-infectious nano-class molecular preparation for animals is prepared through fusing the interleukin-4 gene of pig with the interleukin-6 gene of pig, configuring its eukaryon expression plasmid VPIL 46, preparing chitosan nanoparticles by ion exchange method, dissolving said plasmid in the solution of tripolyphosphate and deacetyl chitosan solution in acetate acid, and proportional mixing. It can be used to immunize the experimental animal by intramuscular injection.

Description

technical field [0001] 1. Biotechnology 2. Molecular Immunology [0002] Specifically including the cloning and fusion of porcine interleukin-4 and 6 genes and the construction of eukaryotic expression plasmids, the molecular packaging of Tibetan pig interleukin-4 and 6 fusion gene eukaryotic expression plasmids by chitosan nanoparticles and its Applied techniques for enhancing immune response and immune protection in animals. Background technique [0003] Improving animal disease resistance defense and immune response ability and improving vaccine protection rate are the most reliable and simple ways to prevent and treat infectious diseases. In view of the fact that traditional drug treatment and routine vaccination can no longer guarantee the healthy and efficient development of modern animal husbandry, animal immunosuppression and the decline of vaccine immune response and protection level have seriously restricted the prevention and treatment of infectious diseases. Th...

Claims

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Application Information

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IPC IPC(8): A61K48/00
Inventor 高荣武梅孟民杰李江凌王泽洲吕学斌李惠付满良吴凯源杨毅沈翼
Owner SICHUAN JINZHAO BIOTECH
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