Method for manufacturing feedstuff protein by solid state fermentation of apple dregs liquid

A feed protein and solid-state fermentation technology, applied in animal feed, animal feed, application, etc., can solve the problems of high equipment investment and production costs, large amount of seed medium consumption, complicated pure culture of bacteria, etc., and achieve high equipment utilization rate , shorten the fermentation time and production cycle, and reduce the effect of equipment investment

Inactive Publication Date: 2005-09-07
NORTHWEST UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method can overcome the disadvantages of the existing single solid-state fermentation to produce feed protein, such as large amount of seed culture medium, complicated pure culture of strains, high equipment investment and production costs, and relatively long fermentation time.

Method used

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  • Method for manufacturing feedstuff protein by solid state fermentation of apple dregs liquid

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Experimental program
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Effect test

Embodiment 1

[0034] Seed medium: 200g of potatoes, 20g of sucrose, some water; the production method is as follows: cut the potatoes into small pieces, add an appropriate amount of tap water to boil for 20-30min, filter, take the filtrate and add tap water to 1000mL.

[0035] Cultivation of seed strains: Take 300mL seed culture medium and put it into a 500mL conical flask and keep it at 121°C for 20min to sterilize. Cultivate at 140 rpm for 36-54 hours to obtain mature seed strains.

[0036] The liquid submerged fermentation medium consists of: dry apple pomace 90%, sucrose 5%, urea 2%, (NH 4 ) 2 SO 42%, KH 2 PO 4 1%. And add 10 times the water of solid material.

[0037] The mixed strains and ratios of the access: Candida utilis: Trichoderma viride: Saccharomyces cerevisiae=4:3:1.5,

[0038] The solid medium consists of: dry apple pomace 75%, bran 20%, urea 2%, (NH 4 ) 2 SO 4 2%, KH 2 PO 4 1%, add water to adjust the initial water content to 60%.

[0039] Fresh and wet app...

Embodiment 2

[0067] Fresh and wet apple pomace is dried at 65°C by a hot air drying line, crushed and sieved, and the 40-mesh raw material is put into a large-scale fermentation tank according to a liquid fermentation medium. The composition of the liquid fermentation medium is: 90% dried apple pomace, 4% sucrose , urea 2%, (NH 4 ) 2 SO 4 2%, KH 2 PO 4 2%, and add 12 times the moisture of the solid material. Simultaneously by the amount of 8% (v / v) access seed tank to cultivate mature mixed strains, the strain ratio of its access: Candida utilis: Trichoderma viride: Fruit wine yeast: Trichoderma korning=2: 1.5 : 6: 3, under 33 ℃, liquid submerged fermentation 36h, then the fermented liquid is poured into the prepared apple pomace solid medium by the amount of 20mL / 100g solid substrate, the composition of culture medium is: dried apple pomace 84% , bran 10%, urea 3%, (NH 4 ) 2 SO 4 2%, KH 2 PO 4 1%. Under the condition of non-sterile water content of 70%, solid-state culture ...

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Abstract

The invention discloses a method of producing feed albumen by solid state fermentation from pomace liquid, whose productive technique is that firstly batching crushed pomance into liquid state fermentation cultivated base, then accessing mixed bacterial with germ all grown to process liquid deep fermentation, finally accessing bacterial liquid after deep fermentation into solid state fermentation cultivated base of pomace to continue fermentation. Compared with single solid state fermentation, the technique mentioned above needs less germ cultivated base, bacterial pure cultivated and reduces the cost. Meantime breed albumen product contains more albumen, nutrition with low cost, and is more fit to product in scale and spreading application for the industry of producing feed albumen by pomace fermentation.

Description

technical field [0001] The invention belongs to a method for producing feed protein by fermenting apple pomace. Background technique [0002] The so-called feed protein (Feeding protein) is single-cell protein (Single cell protein, SCP), also known as microbial protein or cell protein, refers to yeast, fungus, mold, and non-disease bacteria contained in single-celled organisms. The content of protein is as high as 45%-74%, and the combination of various amino acids is reasonable, complete in variety, rich in vitamins and trace elements, and free of cholesterol. These advantages are unmatched by general crop feed. [0003] China is the world's largest apple producer, with an annual output of more than 22 million tons, more than one-third of the world's total. Shaanxi Province is also the largest apple production base in my country. At present, the province's total apple planting area reaches 6.02 million mu, with an annual total output of more than 5.5 million tons, ranking ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37
CPCY02P60/87
Inventor 宋纪蓉徐抗震黄洁马海霞
Owner NORTHWEST UNIV
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