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Method for preparing interferon of white blood cells of dogs

A leukocyte interferon and white blood cell technology, applied in the field of leukocyte interferon, can solve the problems of dog fresh blood sources being easily polluted and production process troubles, and achieve the effects of no toxic side effects, no pollution to the environment, and good curative effect

Inactive Publication Date: 2005-08-31
王明丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the relative species-specificity of canine interferon, and problems such as the contamination of canine fresh blood sources and the production process, the canine interferon for the prevention and treatment of viral diseases in dogs has not been reported yet.

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0021] Preparation process of canine leukocyte interferon

[0022] 1. Prepare anticoagulant (12500 units per milliliter of heparin sodium plus 10 ml of Hank's solution per tube) and antibiotics (kanamycin, final concentration of 100 μg / ml when used).

[0023] 2. Canine whole blood collection. In the animal operation room, aseptically collect canine heart whole blood, collect it into a sterile bottle with anticoagulant and antibiotics added in advance, shake well, and store it in a 4°C refrigerator for later use.

[0024] 3. Prepare chicken Newcastle disease virus (F series) and titrate it.

[0025] 4. Dissolve the RPMI 1640 powder according to the product instructions, filter it through a Chua filter under pressure, and place it in a refrigerator at 4°C for use after the sterility test.

[0026] 5. Preparation of Crude Interferon

[0027] (1) Centrifuge the bottle containing fresh anticoagulant blood at 800-2500rpm for 30min, collect the sallow layer to obtain a suspension r...

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PUM

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Abstract

A process for preparing the leukocyte interferon of dog for preventing and treating canine distemper, canine parvovirus disease, etc includes using chicken Newcastle disease virus (DNV) to induce the leukocyte of health dog, culture, deactivating virus, removing bacteria, separation, purifying and freeze drying to obtain powder injection.

Description

technical field [0001] The invention relates to a leukocyte interferon, in particular to a preparation method of the canine leukocyte interferon used for emergency prevention and treatment of canine distemper, canine parvovirus, canine viral diarrhea and other viral diseases. Background technique [0002] Viral infections such as canine distemper, canine parvovirus, and canine viral diarrhea are relatively common in dogs, and are currently the main infectious diseases that seriously endanger the healthy development of the dog industry. Dogs of all ages are susceptible, especially puppies. Once the dog is infected, it has the characteristics of acute onset, severe clinical symptoms, easy to spread, and high infection rate and mortality, causing huge economic losses to dog breeders. More importantly, pet dogs are in close contact with humans, and some zoonotic diseases pose potential threats to human health. For such viral diseases, there is currently no effective clinical t...

Claims

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Application Information

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IPC IPC(8): A61K38/21A61P31/12C07K14/555C12P21/02
Inventor 王明丽
Owner 王明丽
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