Method for detecting anionic surfactant concentration in waste water using spirogyra
A surfactant, anionic technology, applied in the field of anionic surfactant concentration, can solve the problems of expensive mobile phase, unsuitable sample detection, high technical difficulty of high performance liquid chromatography, etc., achieving obvious symptoms and easy observation and judgment. Effect
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example 1
[0036] The biodegradation efficiency of the anionic surfactant LAS (sodium linear alkylbenzene sulfonate) was measured by the sponge injury symptom method:
[0037] In order to study the biodegradability of anionic surfactants, the surfactant degrading strains were inoculated in an anionic surfactant LAS (linear alkylbenzene sulfonate) solution with an initial concentration of 2000mg / L, and the strains were inoculated in a 250mL conical After the bottle was cultured on a shaker at 37°C for 48 hours, the remaining LAS concentration was determined.
[0038] 1. Preparation of materials
[0039] Anionic surfactant standard sample: sodium linear alkylbenzene sulfonate, analytically pure, purchased from Sigma (U.S.);
[0040] Spirogyra is taken from a ditch not polluted by toxic substances in the suburbs of Changsha. Spirogyra grows well and is in the vegetative growth period. After washing with distilled water, place it in a large beaker (1000 mL) filled with distilled water for ...
example 2
[0057] Determination of Anionic Surfactant Concentration in Wastewater Discharged from a Chemical Plant
[0058] 1. Preparation of materials
[0059] Anionic surfactant standard sample: sodium linear alkylbenzene sulfonate, analytically pure, purchased from Sigma Company (U.S.); Spirogyra: taken from a ditch not polluted by toxic substances in the suburbs of Changsha, Spirogyra grows in good condition and is in the Vegetative growth period. After washing with distilled water, place it in a large beaker (1000 mL) filled with distilled water for later use.
[0060] 2. Determination of non-response critical concentration
[0061] (1) Preliminary determination of the non-response critical concentration of Spirogyra: Dilute the anionic surfactant standard sample LAS in a 50mL small beaker with distilled water to prepare 0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 , 7.0, 8.0mg / L concentration gradient; use tweezers to take about 5g of sponge, absorb the external moisture with absorbent pap...
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