Process for preparing notoginsen triterpenes

A technology of Panax notoginseng saponins and Panax notoginseng, which is applied in the field of preparation of Panax notoginseng saponins, can solve problems such as failure to consider saponin components, difficulty in extracting high purity, poor purity and yield, etc., and achieve saving medicinal materials, The effect of short extraction time and no heating

Inactive Publication Date: 2005-07-06
张平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above preparation method has the following deficiencies: for the poor resolution of this pair of contradictions of purity and yield, the yield declines while the purity improves, and the purity declines while the yield increases, and both can not be well taken into account; macropores Adsorption resin or polyamide or n-butanol extraction is a key step of purification. Due to the limitation of the method itself, it is difficult for the extract to achieve high purity; It is not advisable to purify the extract of Panax notoginseng by technology; each part of Panax notoginseng contains similar saponins, but the content and the ratio of monomer saponins are different.
Chinese Journal of Hospital Pharmacy, 1995, 15 (11): 513], the above method does not take into account that the saponins are easily decomposed and the content is reduced during the long-term heating process in the extraction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] With Radix Notoginseng stem base (reed head, cut mouth) 10kg, pulverize, pass 60 mesh sieves, add the water of 10 times amount, put into ultrasonic extraction tank, carry out ultrasonic vibration extraction, time is 50 minutes, and oscillation frequency is 20kHz, Extract 4 times, combine the extracts, centrifuge at 20000 rpm, pass the centrifuge through the treated D101 macroporous adsorption resin column, first elute with 5 times the column volume of water, and then change to 8 times the column volume 75% ethanol solution was eluted, and the ethanol eluate was collected, concentrated, dried and crushed at 50°C to obtain a powder; the powder was dissolved in 40 times the amount of 50% ethanol, and the particle diameter of the chromatographic column was octane with a diameter of 5 μm. Silane-bonded silica gel is used as a preparative high-performance liquid chromatography system as a filler for separation, eluting with 60% ethanol solution, collecting the eluate rich in n...

Embodiment 2

[0039]Grind 10 kg of Panax notoginseng main root, pass through a 20-mesh sieve, add 6 times the amount of water, put it into an ultrasonic extraction tank, and perform ultrasonic vibration extraction for 20 minutes at an oscillation frequency of 80 kHz. Extract twice, and combine the extracts. Centrifuge at a speed of 12000 r / min, pass the centrifugate through the treated AB-8 macroporous adsorption resin column, first elute with 10 times the column volume of water, and then wash with 4 times the column volume of 95% ethanol solution Remove, collect the ethanol eluate, condense with a rotating scraping film at 50°C, dry, and pulverize to obtain a powder; dissolve the powder with 20 times the amount of 50% ethanol, and use octadecylsilane bonded silica gel with a column particle diameter of 15 μm The preparative high-performance liquid chromatography system used as a filler is used for separation, eluting with 20% ethanol solution, collecting the eluent rich in notoginseng sapon...

Embodiment 3

[0041] Grind 10 kg of Panax notoginseng root, pass through a 40-mesh sieve, add 8 times the amount of water, put it into an ultrasonic extraction tank, and perform ultrasonic vibration extraction for 40 minutes, with an oscillation frequency of 50 kHz, extract 3 times, and combine the extracts , centrifuged at a speed of 16,000 rpm, and the centrifugate was passed through a D101 macroporous adsorption resin column that had been treated, and was first eluted with 8 times the column volume of water, and then eluted with 6 times the column volume of 85% ethanol solution , collect the ethanol eluate, condense it with a rotating scraping film at 50°C, dry, and pulverize to obtain a powder; dissolve the powder with 30 times the amount of 50% ethanol, and use octadecylsilane bonded silica gel with a column particle diameter of 10 μm to obtain The preparative high-performance liquid chromatography system of the filling agent is used for separation, eluting with 40% ethanol solution, co...

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Abstract

The invention discloses a method for preparing a notoginseng triterpene. The notoginseng triterpene is obtained from the caudex or axial root or rootlet or leaf of notoginseng by ultrasonic oscillation extraction, macroporous adsorptive resin passing or water saturated n-butyl alcohol extraction and enrichment, reverse preparation type highly effective liquid chromatographic system purification. The purity of the main active site notoginseng triterpene amounts to 98% above. The notoginseng triterpene has a good action for prevention and treatment of cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine pharmacy, and in particular relates to a preparation method of Panax notoginseng saponins. Background technique [0002] Panax notoginseng is a commonly used traditional Chinese medicine in my country, and the total saponins of Panax notoginseng contained in it are its main effective parts. The role of immune function. The medicinal parts of Panax notoginseng include the stem base (reed head, cut mouth), main root, and branch root (tendon), and the leaves of Sanqi also contain a large amount of saponins. The content of total saponins in each medicinal part Differences. In order to obtain high-purity Panax notoginseng saponins, researchers have made unremitting efforts. The patent document of application number 02158727.2 discloses a method for extracting notoginseng saponin, which is obtained by using a water extract over a polar ADS-3 type adsorption resin column, wherein t...

Claims

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Application Information

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IPC IPC(8): A61P7/02A61P9/10A61P29/00A61P37/04C07J9/00C07J17/00C07J71/00
Inventor 张平
Owner 张平
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