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Method for preparing fixed yeast cell micro capsule

A technology for immobilizing yeast and yeast cells, which is applied in the field of microcapsule preparation to achieve the effects of increased yield, uniform distribution, and good biocompatibility

Inactive Publication Date: 2005-05-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High-power high-voltage pulse microcapsule preparation instrument

Method used

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  • Method for preparing fixed yeast cell micro capsule
  • Method for preparing fixed yeast cell micro capsule

Examples

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example 1

[0022] Example 1: Please refer to the preparation process of the present invention figure 1 . The yeast GS115 in the logarithmic growth phase of the suspension culture was centrifuged to collect the bacteria, and mixed evenly with 15g / L sodium alginate solution. Under the high-voltage electric field of a high-power microcapsule preparation instrument, it was dropped into 100mmol / L calcium chloride solution for gelation reaction for 30 minutes to prepare calcium alginate gel beads with a particle size of 300 μm, and then mixed with 5g / L Chitosan solution film-forming reaction for 15 minutes, 55mmol / L sodium citrate solution was liquefied for 10 minutes after washing with normal saline for 3 times, and chitosan microencapsulated yeast was prepared after washing with normal saline for 3 times, and the thickness of the capsule was 50 μm. The whole preparation process is completed under physiological conditions, and the survival rate of yeast cells is maintained at 100% during the...

example 2

[0023] Example 2: After fully contacting the chitosan solution prepared for the preparation of microcapsule membranes with yeast cells for 30 minutes, the dilution plate method was used to measure its effect on the activity of yeast cells. The number of live yeast cells did not decrease, which confirmed that the chitosan solution had no effect on the activity of yeast cells.

example 3

[0024] Example 3: Place the prepared sodium alginate-chitosan microcapsules and calcium alginate microgel beads containing yeast cells in YPD culture medium, culture in a shaker flask, 28°C, 180rpm, after 24 hours of cultivation, calculate the yeast cell leak rate. The results showed that the cell leakage rate of the chitosan microcapsule group was more than 2.5 times lower than that of the calcium alginate microgel bead group.

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Abstract

The present invention relates to immobilized yeast cell sodium alginate-chitosan microcapsule preparing process. On the basis of original immobilized alginate cell, one great power microcapsule preparing instrument is adopted to make the sodium alginate solution with homogeneously mixed yeast cell under high voltage electrostatic field form homogeneously distributed liquid drops of controllable particle size in 100-500 micron and form micro bead with fixing calcium solution. The surface of the micro bead has one layer of microcapsule chitosan film formed via ion complexing reaction, and calcium in calcium alginate gel is replaced with sodium citrate solution to form liquefied liquid environment inside the microcapsule. The present invention is prepared under mild completely physiological condition of pH 5.0-7.4, 4-30 deg.c and no shearing and vibration. The preparation process has yeast cell activity 100 % maintained and the culture process has cell leakage less than 10 %.

Description

technical field [0001] The invention relates to a preparation method of microcapsules, in particular to a preparation method of sodium alginate-chitosan microcapsules of immobilized yeast cells. Background technique [0002] Yeast cells are the most widely used type of microorganisms in the brewing industry and genetic engineering technology. The study of yeast cell immobilization has a history of nearly 30 years [Document 1: Kierstan M, Bucke C. Theimmobilization of microbial cells, subcellular organelles, and enzymes incalcium alginate gels. Biotechnol Bioeng 1977 Mar;19(3):387-97]. Among the many yeast cell immobilization methods, alginate immobilization is widely used due to its simplicity, easy scale-up, and good maintenance of cell activity [Document 2: Xianzhen Li. The use of chitosan to increase the stability of calcium alginate beads with entrapped yeast cells. Biotechnol. Appl. Biochem. 1996, 23: 269-271]. But at present, there are still many problems in the algi...

Claims

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Application Information

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IPC IPC(8): C12N11/02C12N11/04
Inventor 马小军于炜婷王为
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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