Gel electrochromatophoresis band fast dyeing method

Abstract

The fast gel electrophoresis belt dyeing method includes the following steps: setting gel plate obtained through electrophoresis into dyeing liquid inside non-metal container, and applying microwave radiation for 2-10 min while maintaining the flow of the dyeing liquid and controlling the temperature in 25-60 deg.c. The microwave radiation to promote molecule vibration and raise the temperature can shorten the dyeing time greatly.

Description

Technical field [0001] The invention relates to a protein staining technique in biochemical experiments, in particular to a protein band rapid staining technique in the protein gel electrophoresis determination process. Background technique [0002] In biochemical experiments, the analysis of proteins, nucleic acids, polysaccharides, etc. usually uses gel electrophoresis technology to separate the analysis objects. After electrophoresis separation, the gel plates loaded with protein bands can be identified after staining. There are many methods of protein staining, and Coomassie brilliant blue staining and silver staining are commonly used at present, but no matter which staining method, the staining time will take several hours, or even a day, which is a long time. Although heating, shaking and other means are used, it is still difficult to achieve the purpose of rapid protein staining. [0003] In order to solve the time-consuming problem of the above-mentioned traditiona...

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Problems solved by technology

[0003] In order to solve the time-consuming problem of the above-mentioned traditional protein staining process, the present invention proposes a rapid protein staining method, that is, on the basis of the original protein staining technology, use a special device to irradiate the dyed sample with a certain dose of microwaves according to a certain procedure, so that The dyeing speed is greatly improved, and the time is shortened to a few minutes

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