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Heparin sodium and its preparing process

A technology of sodium heparin and sodium chloride solution, applied in the field of biological medicine, can solve the problems of low yield, polluted environment by waste water, unstable quality of heparin and the like

Inactive Publication Date: 2005-01-19
张国良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the traditional heparin sodium preparation process is extracted from bovine lungs, which not only has high production cost, but also has low yield and the quality of heparin is not stable enough.
Even if some use the preparation process of extracting heparin in the intestinal mucosa, the process is complicated and cannot meet the requirements of industrial production
According to statistics, extracting 1 kg of heparin sodium often requires 2,500 to 3,000 pieces of pig small intestinal mucosa, and a large amount of waste water is generated during the production process to pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Add 700 kilograms of water to 300 kilograms of pig small intestinal mucosa, adjust the pH to 9 with 35% sodium hydroxide, and then add 360 kilograms of 2709 protease at a rate of 1.2 grams per kilogram of pig small intestinal mucosa after heating to 50°C. After stirring for 1.5 hours, add 45 kg of coarse salt, heat up to 85°C, stop stirring, keep warm for 25 minutes, and filter with a cloth bag to obtain the filtrate;

[0020] (2) Add 30 kg of D254 strong basic anion exchange resin to the filtrate, stir and absorb for 2.5 hours, and separate the mother liquor to obtain the exchanged resin;

[0021] (3) Taking the resin after immersion exchange as the amount, wash and stir with a sodium chloride solution of 0.04 moles per liter and a sodium chloride solution of 0.4 moles for 15 minutes, and discard the washing solution to obtain the washed resin;

[0022] (4) Take the immersed and washed resin twice in succession, add 3 moles of sodium chloride solution per liter to reac...

Embodiment 2

[0031] (1) Add 600 kilograms of water to 200 kilograms of pig small intestinal mucosa, adjust the pH to 9.5 with 30% sodium hydroxide, and then add 240 kilograms of 2709 protease at a rate of 1.2 grams per kilogram of pig small intestinal mucosa after heating to 55°C. After heating and stirring for 1.5 hours, add 30 kg of coarse salt, heat up to 80°C, stop stirring, keep for 30 minutes, and filter with a cloth bag to obtain the filtrate;

[0032] (2) Add 26 kg of D254 strong basic anion exchange resin to the filtrate, stir and absorb for 3 hours, and separate the mother liquor to obtain the exchanged resin;

[0033] (3) Take the resin after immersion exchange as the quantity, wash and stir 13 minutes with 0.05 mole sodium chloride solution and 0.5 mole sodium chloride solution per liter, and discard the washing solution to obtain the washed resin;

[0034] (4) Take the resin after immersion and washing as the quantity twice, add 3.5 moles of sodium chloride solution per liter to r...

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PUM

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Abstract

The invention discloses a heparin sodium and its preparing process for extracting sodium heparin from pig small intestine mucous membrane through the steps of, enzymolyzing 2709 proteinases to obtain the liquemine solution, charging sodium chloride, elevating temperature to condensate the animal protein in the pig small intestine mucous membrane, thus resulting ionic exchange for the obtained heparin sodium, performing elution with the strong alkalinity ion exchange resin after washing diluted sodium chloride solution and high concentration sodium chloride solution, subjecting the eluted heparin sodium in alcohol solution for settlement and drying to obtain the liquaemin sodium crude product, acidifying the soluble heparin crude product to remove acidic proteins, then performing purification operation of double oxidization.

Description

Technical field [0001] The invention relates to the technical field of biological medicines, in particular to a heparin sodium biological medicine and other preparation methods. Background technique [0002] Heparin is a natural anticoagulant drug, which has the effects of anticoagulation and prevention of thrombosis. It has extremely high clinical value for patients with nephropathy, acute myocardial infarction, and prevention of blood coagulation during blood transfusion. In recent years, heparin has excellent curative effects in the treatment of cerebrovascular diseases, fulminant meningococcal sepsis and other diseases. However, this heparin is widely distributed in the tissues of animal organs, such as intestinal mucosa, duodenum, lung, liver, pancreas, placenta and blood. Heparin, like most mucopolysaccharides, exists in the body as a protein-bound complex, and the anticoagulant properties of this complex increase with the removal of its protein activity. Obviously, the lev...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 张国良
Owner 张国良
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