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Endotoxin adsorbing agent and preparing method thereof

An adsorbent and endotoxin technology, applied in the fields of biochemical separation and biomedical materials, can solve the problems of polymyxin B, which is expensive, nephrotoxic, unsuitable for promotion, toxic and side effects, etc., and achieves high recognition ability, adsorption capacity, and low cost Low, less toxic and side effects

Inactive Publication Date: 2004-09-15
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polymyxin B is expensive and has nephrotoxicity. If it falls off from the carrier, it will have toxic side effects, and the above method needs to separate plasma from blood cells and perform plasma perfusion. The cost is very expensive and it is not suitable for domestic promotion.

Method used

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  • Endotoxin adsorbing agent and preparing method thereof
  • Endotoxin adsorbing agent and preparing method thereof
  • Endotoxin adsorbing agent and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1-1 Preparation of polymethacrylate carrier

[0054] In a three-neck flask equipped with a stirrer, a reflux condenser, and a thermometer, 8.1 g of methyl methacrylate containing 0.414 g of benzoyl peroxide and 18.8 g of divinylbenzene mixed solution were added to a mixture containing 20% ​​chlorinated Add 5-8 drops of 0.1% methylene blue to the aqueous phase (2-4 times the volume of the organic phase) of sodium and 0.5% gelatin. Adjust the stirring speed until the size of the liquid beads is suitable and the particle size is uniform, then raise the temperature to 67°C, and polymerize for two hours; then raise the temperature to 75°C, and polymerize for two hours; then raise the temperature to 85°C, and polymerize for two hours; then raise the temperature to 95°C, and keep the temperature for four hours Hours, the carrier PMMA was prepared. Repeated scrubbing with hot water, extraction with absolute ethanol for 8 hours, drying, and sieving to collect carriers with a pa...

Embodiment 2

[0090] 2-1 Preparation of Chitosan Resin

[0091] To 5% chitosan acetic acid aqueous solution (5g chitosan+3ml glacial acetic acid+100ml water), add 1g Span 80 dissolved in 300ml toluene solution, and stir for 1.5 hours. 50ml of 50% glutaraldehyde aqueous solution was slowly added dropwise, the temperature was raised to 50°C, and the temperature was kept constant for 1.0 hour. Aqueous sodium hydroxide solution was added to adjust the pH of the reaction system to be slightly alkaline. Raise the temperature to 80°C and keep the temperature constant for 4 hours. The product is extracted with absolute ethanol, washed with water, weighed, and sieved to collect the carrier with a particle size of 280-900 μm. NaBH under alkaline conditions at room temperature 4 Restore for 24 hours, wash and set aside. The content of remaining amino groups was measured by acid-base titration method to be 101.3-120.7 μmol / g.

[0092] 2-1 Epoxy Activation

[0093] Add 1g of chitosan resin, 5ml of...

Embodiment 3

[0101] 3-1 Preparation of spherical agar carrier

[0102] Place a 500ml three-necked flask in a 60°C water bath, add 100ml of toluene, 50ml of carbon tetrachloride, and 0.5ml of Tween 80 into the bottle, and stir evenly. Weigh 4 grams of agar powder and add 30ml of distilled water, heat and melt, pour the melted agar into the toluene-carbon tetrachloride system, stir, and disperse the agar solution into droplets of suitable size in the organic phase. Cool to room temperature, pour out the organic solvent in the upper layer, screen the agar balls with a particle size of 280-900 μm, wash with water, transfer to an Erlenmeyer flask, and store in a wet state at 4°C.

[0103] 3-2 Epichlorohydrin activation

[0104] Take 20.0 grams of agar balls, add 29.6 milliliters of 2M sodium hydroxide, then add 13.3 milliliters of epichlorohydrin, react at 40 ° C for 2 hours, wash with water until neutral, and thoroughly wash off the remaining epichlorohydrin, Epoxy-activated gel balls were o...

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Abstract

The invention relates to an endotoxin sorbent as well as its making method, using natural or synthetical high molecule as carrier and dimethyl amine as ligand, and linked with oxhydryl at beta bit of the dimethyl amine, and its amidocyanogen content is 10-130 mu mol / g. Because electrovalent bond, hydrogen bond and eight ring can form at the same time between this structure and the endotoxin and the hydrophobic chain of the endotoxin and the sorbent is on the same side of the eight ring, and it has endotoxin -identifying and -absorbing ability. It can eliminate the endoxtoxin in biochemical products and in patient's blood.

Description

technical field [0001] The invention relates to the fields of biochemical separation and biomedical materials, in particular to an endotoxin adsorbent and a preparation method thereof. Background technique [0002] Endotoxemia is caused by the release of endotoxins from the outer cell wall of Gram-negative bacteria into the blood, and can occur in various disease processes [Wang Jinda, China Critical Care Medicine, 1998, 10(10): 578] , such as: large area burns, severe hepatitis, liver cirrhosis and other diseases, the immune function of the body is severely impaired, and can cause a series of serious pathological changes such as shock, disseminated intravascular coagulation, multiple organ failure, and eventually lead to organ necrosis , irreversible shock and death. In the United States, more than 100,000 people die from this disease every year (Skelter et al, Arch. Microbiol. 1995, 164, 383-389.), and there is no effective treatment method in clinic. How to timely and e...

Claims

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Application Information

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IPC IPC(8): A61M1/34A61M1/36B01J20/22B01J20/28
Inventor 袁直俞玫王慧彦李纪红侯光辉王孝杰张文升
Owner NANKAI UNIV
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