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Vero cell fick-borne encephalitis inactivated vaccine

A technology of forest encephalitis and forest encephalitis virus, applied in the field of biopharmaceuticals, can solve the problems that forest encephalitis vaccines have not been reported.

Inactive Publication Date: 2004-07-21
长春生物制品研究所有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vero cell poliomyelitis inactivated vaccine, polio vaccine, Vero cell rabies vaccine have been cultivated and developed in France successively and approved for production, and there are reports on the application of Vero cells to prepare vaccines, but about the application of Vero cells to prepare forest encephalitis vaccine research has not yet been reported

Method used

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  • Vero cell fick-borne encephalitis inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Take the Vero cell bottle for production that has grown into a monolayer, discard the cell growth solution, add 0.1-0.5% trypsin at pH 7.6-8.0, and digest at 37°C for 15-40 minutes, discard the trypsin solution, and use Gently disperse the cells in the fresh cell growth solution, suspend them in the cell growth solution containing 6-8% calf serum MEM solution, prepare the cell suspension, shake it evenly and distribute it in 3 liter culture flasks, 200ml- 600ml / bottle. The cell culture flasks were placed in a 37°C constant temperature room for rotary culture until the cells grew into a uniform and dense monolayer.

[0064] Select a single-layer cell bottle with uniform and dense cell growth, discard the cell growth solution, rinse with Earle's solution for 2-3 minutes, and then infect the vaccine virus strain virus seed (the infection dose of the virus seed is that the multiplicity of infection is 0.02-0.5, and the virus seed The dilution solution is 0.1-0.2% human alb...

Embodiment 2

[0067] Take the Vero cell bottle for production that has grown into a monolayer, discard the cell growth solution, add 0.1-0.5% trypsin at pH 7.6-8.0, and digest at 37°C for 15-40 minutes, discard the trypsin solution, and use Gently disperse the cells in the fresh cell growth solution, suspend them in the cell growth solution containing 6-8% calf serum MEM solution, prepare the cell suspension, shake it evenly and distribute it in 3 liter culture flasks, 200ml- 600ml / bottle. The cell culture flasks were placed in a 37°C constant temperature room for rotary culture until the cells grew into a uniform and dense monolayer.

[0068]Select cell growth uniform, compact single-layer cell bottle, discard the cell growth solution and infect the virus seed of the vaccine strain (the infection dose of the virus seed is that the multiplicity of infection is 0.02-0.5, and the virus seed dilution is 0.5-2.0% calf serum MEM solution), add the virus seed solution to the cell bottle to be in...

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Abstract

An deactivated vero cell vaccine for forest encephalitis virus is composed of the deactivated forest encephalitis virus and vaccine adjuvant. Its preparing process includes inoculating the forest encephalitis virus to vero cells, culturing to obtain the culturing liquid, purifying, and preparing said vaccine. Its advantages are high purity, high immunizing effect and high safety.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to an inactivated Vero cell forest encephalitis vaccine and a preparation method thereof, in particular to a method for industrially preparing an inactivated Vero cell forest encephalitis vaccine and the vaccine prepared by the method. Background technique [0002] Forest encephalitis is caused by forest encephalitis virus. Ticks are the main source of infection. It is a natural foci acute infectious disease mainly invading the central nervous system. The case fatality rate is 20-30%. Healthy viral infectious disease. [0003] Vaccines for the prevention of forest encephalitis. Successively experienced the preparation of forest encephalitis inactivated vaccines with mouse brain, chicken embryo, hamster kidney and other matrices. At present, the inactivated vaccine of forest encephalitis of hamster kidney cells is used in my country; the inactivated vaccine of forest en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61P31/12C12N7/00
Inventor 宋宗明韩亮赵红丽洪成龙岳立广刘双军赵丽红钱依群惠连王晓宏赵建郝富勇王伟
Owner 长春生物制品研究所有限责任公司
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