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Figwort polysaccharide and its production process and use

A production method and technology of Scrophulariaceae, applied in the field of homologous polysaccharide mixtures, to achieve high yield and simple extraction and separation methods

Inactive Publication Date: 2004-04-07
SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Literature reports, [Zhang Wenjie et al., Yunnan Botanical Research, 16(4), 407(1994)], [Li Yiming, Jiang Shanhao, Gao Wenyun, Zhu Dayuan, Acta Pharmaceutica Sinica, 34(6), 448(1999)], [Zou Chenting, Yang Xiuwei, Chinese Herbal Medicine, 31(4), 241(2000)], its chemical components mainly include alkaloids, flavonoid glycosides, sterols, amino acids, fatty acids, volatile oils, carotene, iridoid glycosides and trace elements, etc. Studies on polysaccharides in Scrophulariaceae have not been reported

Method used

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  • Figwort polysaccharide and its production process and use
  • Figwort polysaccharide and its production process and use
  • Figwort polysaccharide and its production process and use

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Wash and dry 500 g of the crude drug Scrophulariaceae Scrophulariae, crush, soak in 5 liters of deionized water at room temperature for 24 hours, and filter. Continue soaking the residue in 2.5 liters of deionized water for 12 hours, filter, combine the filtrates, and concentrate under reduced pressure at 60°C to 1-2 liters. Then precipitate with 95% ethanol, the final concentration of ethanol is 80%, and precipitate for 24 hours. Centrifuge, dissolve the precipitate with 1.25 liters of water, centrifuge, and dialyze with deionized water for 48 to 72 hours. Concentrate under reduced pressure at 60°C and freeze-dry to obtain 8.9 g of total sugars from Scrophulariaceae. Add 2 liters of water to dissolve, add active carbon to treat, remove three times, then concentrate under reduced pressure, centrifuge, and freeze-dry to obtain 3.0 g of total sugar of Scrophulariaceae with a purity of 80%.

Embodiment 2

[0033]Wash and dry 500 g of crude drug Scrophulariaceae Scrophulariae, crush, soak in 5 liters of deionized water at room temperature for 36 hours, and filter. The residue was soaked in 2.5 liters of deionized water for 12 hours, filtered, and the combined filtrates were concentrated to 2 liters under reduced pressure at 50°C. Then precipitate with 95% ethanol, the final concentration of ethanol is 82%, and precipitate for 48 hours. Centrifuge, dissolve the precipitate with 2 liters of water, centrifuge, and dialyze with deionized water for 72 hours. Concentrate under reduced pressure at 50°C and freeze-dry to obtain 9.5 g of total polysaccharides from Scrophulariaceae. Add 2 liters of water to dissolve, add active carbon to treat, remove three times, then concentrate under reduced pressure, centrifuge, and freeze-dry to obtain 3.1 g of total polysaccharides of Scrophulariaceae Scrophulariae with a purity of 80%.

Embodiment 3

[0035] Scrophulariaceae total sugar was purified by DEAE-Cellulose column chromatography with a purity of 85-90%, and then SephadexG-25 and Sephadex G-75 column chromatography to obtain Scrophulariaceae polysaccharide SnPS-1 with a purity of 99.6%. Specific separation method: weigh 100 mg of total sugar from Scrophulariaceae, dissolve in 1-2 mL of distilled water, and centrifuge (4500 r / min, 10 min). Put the supernatant on a Sephadex G-25 column (1.5cm×100cm), elute with 0.1mol / L NaCl at a flow rate of 0.5mL / min, collect 3-5mL in each tube, and about 37-47 tubes is the absorption peak. After collection, it is purified by Sephadex G-75 column and freeze-dried to obtain pure polysaccharide SnPS-150mg.

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Abstract

The present invention is polysaccharide extracted from Chinese medicinal material figwort and its production process and use. Figwort is processed to obtain total saccharide through water soaking, organic solvent precipitation, water dissolution of the precipitate, dialysis of supernatant or membrane separation, concentration and drying; and figwort total saccharide is further processed through active carbon treatment and column chrumatography to obtain figwort polysaccharide SnPS-1. The chemical structure of SnPS-1 includes main chainsí·2)Gal(1í·and í·2)Araf(1í·; small amount of sacharide chains í·6) Manp (1í·, í·5) Araf(1í·, í·3) Glcp (1í· and í·3) Rhap (1í·; thimbleful branch saccharide í·2ú¼4ú® Xylp(1í·; and terminal saccharide Galp (1í·, and this shows that SnPS-1 is heteropolysaccharide with straight chain as main component and less branches. Bioactivity experimant shows that the polysaccharide has cerebral ischemica protecting function and no toxic side effect.

Description

technical field [0001] The invention relates to a polysaccharide homologous mixture extracted from Chinese medicinal materials, in particular to Scrophulariaceae polysaccharide SnPS-1 extracted from crude drug Scrophulariaceae Scrophulariae, its production method and application. technical background [0002] Scrophulariaceae is a traditional Chinese medicinal material, which has the effects of "cooling blood and nourishing yin, reducing fire, promoting body fluid, and detoxifying". Literature reports, [Zhang Wenjie et al., Yunnan Botanical Research, 16(4), 407(1994)], [Li Yiming, Jiang Shanhao, Gao Wenyun, Zhu Dayuan, Acta Pharmaceutica Sinica, 34(6), 448(1999)], [Zou Chenting, Yang Xiuwei, Chinese Herbal Medicine, 31(4), 241(2000)], its chemical components mainly include alkaloids, flavonoid glycosides, sterols, amino acids, fatty acids, volatile oils, carotene, iridoid glycosides and trace elements, etc. Studies on polysaccharides in Scrophulariaceae have not been report...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P9/10C08B37/00
Inventor 田庚元邓军娥
Owner SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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