Process for preparing grist powder contained chrome-enriched yeast
A technology of chromium-enriched yeast and malt powder, applied in the direction of fungi, etc., can solve the problems of low content, inconvenient transportation and storage, unfavorable waste liquid treatment, etc.
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Embodiment 1
[0014] 1. Preparation of malt powder
[0015] 1) Select 25Kg of wheat grains, place them in a chromium chloride solution with a concentration of 50-1500 μg / ml for cultivation, the ratio of wheat to liquid is 1:0.5-0.7, and the cultivation temperature is 10-25° C., and cultivate for 72 hours;
[0016] 2) Stir once every 2 hours to keep the solution evenly absorbed;
[0017] 3) When the malt grows 2-4mm, take it out and wash it 2-3 times, and let it dry naturally;
[0018] 4) Dried malt is crushed, passed through a 60-80 mesh sieve, and set aside.
[0019] 2. Preparation of yeast
[0020] 1) using wort to make the culture medium of yeast cells, the sugar content is 9-11 Pauline medium, and chromium chloride is added in the culture medium;
[0021] 2) Inoculating the yeast with a volume ratio of 1:1 between the culture medium and the yeast, the chromium concentration is 5-100 ppm, and culturing at 15-30° C. for 24 hours;
[0022] 3) The chromium-enriched yeast is separated by...
Embodiment 2
[0028] 1. Preparation of malt powder
[0029] 1) Select 25Kg of wheat grains, place them in a chromium sulfate solution with a concentration of 50-1500 μg / ml for cultivation, the ratio of wheat to liquid is 1:0.5-0.6, and the cultivation temperature is 10-25°C for 48 hours;
[0030] 2) Stir once every 2 hours to keep the solution evenly absorbed;
[0031] 3) When the malt grows 2-4mm, take it out and wash it 2-3 times, and dry it at 60-80°C;
[0032] 4) Dried malt is crushed, passed through a 60-80 mesh sieve, and set aside.
[0033] 2. Preparation of yeast
[0034] 1) make the culture medium of yeast cell with basic culture medium, add chromium sulfate in culture medium;
[0035] 2) Inoculating the yeast with a volume ratio of 1:1 between the culture medium and the yeast, the chromium concentration is 5-100ppm, and culturing at 15-30°C for 48 hours;
[0036] 3) The chromium-enriched yeast is separated by centrifugation, the residual chromium compound outside the yeast cel...
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