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Recombinant human fatty acid oxidation protein, its production method and application

A fatty acid oxidation and oxidation protein technology, applied in the field of recombinant human fatty acid oxidation protein and its production and use, can solve problems such as affecting the absorption or metabolism of nutrients, increasing energy consumption, etc.

Inactive Publication Date: 2004-02-25
HUNAN YINGCHENG LIFE SCI INST
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] In recent decades, European and American scholars have devoted themselves to the research and development of obesity treatment drugs. Weight loss drugs can be roughly divided into three development directions according to their mechanism of action: (1) suppress appetite; (2) affect the absorption or metabolism of nutrients; (3) increased energy expenditure

Method used

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  • Recombinant human fatty acid oxidation protein, its production method and application
  • Recombinant human fatty acid oxidation protein, its production method and application
  • Recombinant human fatty acid oxidation protein, its production method and application

Examples

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Embodiment Construction

[0020] 1. cDNA clone of recombinant human fatty acid oxidation protein.

[0021] 1. Full-length human fatty acid oxidation protein is obtained from human adipose tissue cDNA library by reverse transcription polymerase chain reaction (RT-PCR), and its upstream and downstream primers are respectively:

[0022] 5′ATG CTG TTG CTG GGA GCT GTT CTA

[0023] 5′ TCA GTT GGT TGT CAT GGT AGA GAA

[0024] 2. Synthesis of first-strand cDNA

[0025] Take 0.5 μg of human adipose tissue cDNA library, add downstream primers, incubate at 65°C for 10 minutes, quickly add to Boehringer first-strand cDNA synthesis system and mix well, place at room temperature for 10 minutes, then incubate at 42°C for 10 minutes, and carry out reverse transcription reaction. Inactivate at 96°C for 10 minutes after completion.

[0026] 3. Polymerase Chain Reaction (PCR)

[0027] Take 2 μl of the above reaction product as a template, add upstream primers, and perform polymerase chain reaction (PCR)...

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Abstract

The present invention relates to a recombinant human fatty acid oxidation protein, its production and application. The protein is formed from 244 amino acids, and is composed of collagenic structuraldomain of N-end and spheriodal structural domain of C-end with biological activity, it also contains the nucleotide sequence for coding human fatty acid oxidation protein, and the spheroidal structural domain of C-end contains cDNA sequence.

Description

technical field [0001] The present invention relates to a recombinant human fatty acid oxidized protein and its production method and application, which is to clone the cDNA sequence of human free fatty acid oxidized lipoprotein and its C-terminal globular domain by the polymerase chain reaction (Polymerase Chain Reaction, PCR) method , a method for producing recombinant human free fatty acid oxidation protein and its C-terminal globular domain by genetic engineering technology. The recombinant protein and its globular domain have clear application value in diseases of obesity and glucose metabolism disorder. Background technique [0002] Over the past two decades, obesity has become more and more common. People have realized that obesity is not caused by overeating, but a disease caused by disorders of appetite and energy regulation caused by factors such as genetics, environment, and dietary structure. [0003] At present, it is known that obesity is significantly related...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/17A61K38/17A61P3/04A61P3/10C07K14/435C07K14/47C12N15/12C12N15/63C12P21/02
Inventor 宁云山肖灿鹏隋杰卢捷龚平杨浩
Owner HUNAN YINGCHENG LIFE SCI INST
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