Method for creating model of transplanting testis of rat

Abstract

A method for creating a rat testis transplantation model includes cutting off the blood vessels of left testis including the left arteria spermatica, left vena spermatica, and the abdominal aorta andleft iliac vein, ligating the far end of left iliac vein, sleeving Cuff tubes to another three broken ends, annular ligation, embedding them in incised abdominal aorta and left iliac vein of receptorrat, and removing both testes of receptor rat. Its advantages are simple operation, high successful rate, and high reliability.

Description

technical field [0001] The invention relates to an animal model, mainly relates to the construction of an organ transplant animal model, in particular to a method for constructing a rat testis transplant animal model, which is suitable for application in the field of organ transplant and reproductive research. Background technique [0002] Rat testicular transplantation model is an important method to study the physiological function and spermatogenic function of testicular transplantation after testicular transplantation. Although the surgical method has been introduced abroad, it is difficult to make the model and has a high failure rate due to the very thin blood vessels in rats. [0003] Lee et al first reported in 1971 that the donor abdominal aorta was used as the testicular artery pedicle, which was anastomosed end-to-side with the recipient abdominal aorta. It became a blood storage pool, where the blood flow formed a vortex, leading to thrombus formation; after that...

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Problems solved by technology

[0002] Rat testicular transplantation model is an important method to study the physiological function and spermatogenic function of testicular transplantation after testicular transplantation; although the surgical method has been introduced abroad, it is difficult to make the model and the failure rate is high due to the very thin blood vessels in rats

[0003] Lee et al first reported in 1971 that the donor abdominal aorta was used as the testicular artery pedicle, which was anastomosed end-to-side with the recipient abdominal aorta. It became a blood storage pool, where the blood flow formed a vortex, leading to thrombus formation; after that, Goldsein and Sunlee made improvements, and under the microscope, the abdominal aortic valve including the right testicular artery and vein and the inferior vena cava valve were anastomosed end-to-side to the abdominal aorta and inferior vena cava of the recipient, but they only used the right testis whose testicular artery and vein run parallel and flow into the abdominal aorta and inferior vena cava respectively, so the proportion of rats that met the requirements was small. Moreover, the intraoperative anastomosis technique is difficult, time-consuming, and difficult to promote; therefore, it is very important to explore a rat testis transplantation model with a simple method and a high success rate for experimental research

[0004] There are some defects in the model building method reported in the previous literature: ①Lee et al. used the donor abdominal aorta as the testicular artery pedicle, and after the blood flow was opened, the abdominal aorta used as the testicular artery pedicle actually became a blood storage pool , where the blood flow forms a vortex, leading to thrombus formation; ②Goldsein and Sunlee only used the right testis whose testicular artery and vein run parallel and flow into the abdominal aorta and inferior vena cava respectively, so the proportion of rats that met the requirements was small (about 22.4%), and the intraoperative anastomosis technique is difficult, time-consuming and difficult to popularize

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