Diethylstilbestrol enzyme linked immunoassay reagent box and determination

Abstract

A chemical reagent box applies enzyme linked immunosorbent assay method to detect diethylstilbestrol. The cony IgG sensitizer is enveloped on the enzyme scale plate and the two are reacted through hatching, washnig, sample diluent and enzyme scale antigen adding after diethyl stilbestrol sensitizer is added. The detection is finished by washing, adding enzyme-substrate material to display coloure and using enzyme scale meter to detect A 450 nm.

Description

technical field [0001] An enzyme-linked immunoassay kit for diethylstilbestrol and a detection method thereof are used for detecting the content of diethylstilbestrol in animal foods. The invention belongs to the technical field of enzyme-linked immunoassay. Background technique [0002] Diethylstilbestrol (Diethylstilbestrol) is a synthetic hormone drug that can be applied clinically to the treatment of menopausal syndrome. In recent years, "food tonic" health products have gradually become a trend to replace medicines, and more and more people accept them. Some medicines are added to the feed of poultry and livestock in order to contain these trace medicines in their eggs and meat, so as to achieve Replace medicine with food. [0003] The determination and instrumental analysis methods of diethylstilbestrol and some other steroid hormones in beef and animal blood have been reported in the literature, but the determination of diethylstilbestrol in eggs is rarely reported....

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Problems solved by technology

[0003] The determination and instrumental analysis methods of diethylstilbestrol and some other steroid hormones in beef and animal blood have been reported in the literature, but there are few reports on the determination of diethylstilbestrol in eggs

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