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Production of organic phosphorous chemicals decomposing enzyme by silkworm bioreactor

An organophosphorus pesticide, silkworm technology, applied in microorganisms, hydrolase, biochemical equipment and methods, etc., can solve the problem that the expression amount is not yet reached

Inactive Publication Date: 2003-11-19
安徽固源生物工程有限责任公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0009] In 1985, Sendar et al. (Bio / technol., Vol.3, p567-568, 1985) first tried to express organophosphate digestion enzymes in Escherichia coli. Although the expressed product had biological activity, the expression level was not as high as that of the original natural strain. one tenth

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  • Production of organic phosphorous chemicals decomposing enzyme by silkworm bioreactor
  • Production of organic phosphorous chemicals decomposing enzyme by silkworm bioreactor
  • Production of organic phosphorous chemicals decomposing enzyme by silkworm bioreactor

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Embodiment Construction

[0024] experiment one

[0025] 1. Bacterial strains, virus strains and vectors Escherichia coli strains E.coli TG1 and DH 5a Purchased from Promega Company; the carrier pYY-01 carrying the organophosphorus pesticide digestion enzyme gene (Chinese invention patent, application number: 01110725.1) was preserved by Anhui Guyuan Bioengineering Co., Ltd., and the transfer plasmid pBacPAK6 was purchased from Clontech Company, silkworm cell line BmN , Bombyx mori nuclear polyhedrosis virus parent strain Bm-BacPAK6 (gal + ) was kindly provided by Dr. Susumu Maeda, University of California, USA. The silkworm variety is JY1, donated by the Key Open Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Institute of Sericulture, Chinese Academy of Agricultural Sciences.

[0026] 2. Enzymes and kits Restriction enzymes and ligases are products of Boehringer.

[0027] 3. Biochemical reagents IPTG, X-Gal, SDS and sodium phytate are products of Sigma. Ammo...

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Abstract

A process for preparing the zymolase of agricultural organophosphorus chemical by use of silkwork bioactivator includes such steps as recombining said zymolase gene with baculovirus vector, infecting host silkworm, expressing of said zymolase, collecting the body fluid of young silkworm or pupa containing said zymolase, homogenizing, and preparing enzyme preparation.

Description

technical field [0001] The present invention relates to the fields of genetic engineering and enzyme preparations, in particular to a method for producing organophosphorus pesticide-digesting enzymes with a silkworm bioreactor, through which the organophosphorus-pesticide-digesting enzymes can be produced in large quantities and at low cost for use in fruits, Enzymatic degradation of residual pesticides in vegetables and other agricultural products, production of agricultural products without pesticide pollution, and can also be used for decomposition and purification of residual pesticides in soil, water and other environments. . Background technique [0002] Organophosphorus is the main category of pesticides today. It has been mass-produced and widely used at home and abroad. At present, there are hundreds of organophosphorus pesticides in the world. There are about 30 kinds of organophosphorus pesticides used in my country, including insecticides, Herbicides, fungicides,...

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Application Information

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IPC IPC(8): A01K67/04C12N7/01C12N9/16C12N15/55C12N15/866
Inventor 孙跃军张志芳姚斌孙齐军
Owner 安徽固源生物工程有限责任公司
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