Protein extracting and separating method from defatted cotton dregs
A protein and absorbent cotton technology, applied in the field of protein extraction and separation, can solve the problems of difficulty in producing protein, huge production line equipment, deepening protein color, etc., and achieve the effects of reasonable equipment layout, low cost and less protein loss.
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Embodiment 1
[0016] Embodiment 1 comprises the steps in the following order: degreased cotton meal is dissolved in alkali and centrifuged once to obtain solid phase and supernatant (I), the temperature during alkali dissolution is 50 ° C, and the pH value is 9, and the solid phase is dried to obtain feed Protein, supernatant (I) is subjected to acid precipitation, secondary separation, neutralization, homogeneous sterilization, and spray drying to obtain isolated protein. The pH value is 4.5 during acid precipitation, and the pH value is 7 during neutralization. The homogeneous sterilization is instantaneous sterilization , the temperature is 125°C.
Embodiment 2
[0017] Example 2, the difference between Example 2 and Example 1 is that the temperature during alkali dissolution is 35°C, the pH value is 12, the pH value during acid precipitation is 3.5, and the pH value during neutralization is 6.5, and the homogeneous sterilization is instantaneous sterilization. The temperature is 120°C.
Embodiment 3
[0018] Example 3, the difference between Example 3 and Example 1 is that the temperature during alkali dissolution is 65°C, the pH value is 8, the pH value during acid precipitation is 5.5, and the pH value during neutralization is 7.5, and the homogeneous sterilization is instantaneous sterilization. The temperature was 140°C.
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