Method for transferring exogenous gene into animal cell

A technology for exogenous genes and animal cells, which can be applied to other methods of inserting foreign genetic materials, using microinjection methods, and using vectors to introduce foreign genetic materials, etc.

Inactive Publication Date: 2003-03-05
中国科学院发育生物学研究所
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former requires the pronucleus of the fertilized egg to have a certain degree of visibility (this is very difficult in quite a few animals) and the operator has a relatively high operating technique (not easy to master), and it is easy to cause nuclear damage, resulting in a very low birth rate and failure of genetic modification; The latter is unstable and poorly repeatable
However, the transfection method of in vitro cultured cells has always been troubled by the low transfection rate, and can only be used for cytological observation experiments, and cannot be used for other research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for transferring exogenous gene into animal cell
  • Method for transferring exogenous gene into animal cell
  • Method for transferring exogenous gene into animal cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Our laboratory uses the mouse histone H2AZ (mH2AZ) as a nuclear localization signal to promote the integration of the transgenic construct bovine α-s1-casein-human G-CSF expression vector on the host chromosome, that is, the target gene expression construct and the mH2AZ gene The expression components are co-injected into the fertilized egg cytoplasm, and the target gene expression components are brought into the prokaryon by the directional migration of nuclear localization proteins. The results of this example show that the method used can bring the target gene expression components into the host cell nucleus to obtain transgene integrated mice, and this method is more convenient, practical, and easy to promote, and can bypass the bottleneck of pronuclear injection technology and avoid fertilized eggs. A large number of injuries, promote the widespread application of transgenic animal technology. Therefore, this transgenic approach can completely replace the tradition...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The method for transferring exogenous gene into animal cell includes the following steps: using target gene to construct target gene expression vector, using nuclear localization protein gene to construct nuclear localization protein gene expression vector and transferring the obtained target gene expression vector and nuclear localization protein gene expression vector into the cytoplasm of cultured cell or cytoplasm of animal cylula by means of cotransfection or co-injection or electroporation.

Description

technical field [0001] The invention relates to a method for transferring foreign genes into animal cells. technical background [0002] As a technical platform, transgenics can not only be used to prepare mammary gland bioreactors, but also have very important significance in disease model animals, improved animal breeds, and the study of individual development mechanisms at the molecular level. As a bioreactor, transgenic animals can produce medicinal and rare proteins that cannot be obtained by other production methods, and have the characteristics of high efficiency, low cost and more natural products. Its potential economic and social value has been valued by countries all over the world. However, the production of transgenic animals on a large scale has remained difficult for more than a decade. One of the major hurdles is the bottleneck of transgenic methods. [0003] At present, there are mainly two methods for the preparation of transgenic mammals: pronuclear mic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63C12N15/64C12N15/89
Inventor 张靖溥李光三杜淼
Owner 中国科学院发育生物学研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap