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Collector for nucleic acid analysis and its application method

A collection device and nucleic acid analysis technology, which is applied to biochemical equipment and methods, and microbial measurement/inspection, and can solve problems such as pollution, high false positive and false negative rates, and poor repeatability

Inactive Publication Date: 2002-08-21
SHANDONG TUMOR HOSPITAL
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Problems solved by technology

[0004] The traditional gene analysis methods are slab gel electrophoresis and capillary electrophoresis. Although slab gel electrophoresis can be used to recover and clone gene fragments and sequence them, the operation steps are cumbersome, time-consuming, poor in repeatability, and have false positives and false negatives. higher rate
Capillary electrophoresis has a higher resolution than slab gel electrophoresis, but it cannot recover the samples in the same injection. The possible recovery position of the next injection can only be set according to the electrophoresis results of the previous injection (inferred based on time). The standard comes from non-current electrophoresis results, so it is prone to errors, impure and contaminated recovered samples, and cannot perform real-time monitoring of sample recovery
[0005] At present, there are many methods for nucleic acid fragment analysis and research using nucleic acid analyzers, but in nucleic acid analysis, the instrument and method for simultaneous analysis and collection of nucleic acid differential fragments by capillary electrophoresis has not been registered so far through online patent searches. There is no relevant report in the literature search

Method used

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  • Collector for nucleic acid analysis and its application method
  • Collector for nucleic acid analysis and its application method
  • Collector for nucleic acid analysis and its application method

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Embodiment 1

[0044] Embodiment 1: In this embodiment, the preparation and dosage of the buffer solution are the same as the conventional nucleic acid fragment analysis specifications. The laser 7 is an argon ion laser with a wavelength of 488nm and a power of 20mW. The capillary tubes 11 and 12 of the sample collection passage are 7 cm in length. The analysis channel capillary 4, 5 is 47cm in length.

[0045] (1) Take 0.5 g of normal colon tissue and familial colon polyposis tissue, respectively extract RNA by conventional methods, and then take 20 ng of RNA respectively and use a conventional FAM fluorescein-labeled kit to perform mRNA differential display polymerase chain reaction (DD- PCR) amplification, the amplified product is for use;

[0046] (2) Perfuse capillaries 4, 5, 11, and 12 in a conventional manner with POP-4 gene scanning gel from ABI;

[0047] (3) The above two mRNA DD-PCR amplification products labeled with conventional FAM fluorescence were put into two sample tubes ...

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Abstract

The present invention is a kind of collector for nucleic acid analysis and its application method. The collector consists of an integral double-path capillary with sample analyzing path and sample collecting path, three detecting windows in the analyzing path and a sample collecting pipe. The collector is used in automatic fast analysis of nucleic acid segment and high percision and high purity automatic fast collection of different nucleic acid band. The collector has unique structure, and can be used through combining with traditional capillary nucleic acid nucleic acijd analysis technology, such as DNA sequencing instrument technology.

Description

(1) Technical field [0001] The invention relates to a nucleic acid analysis instrument and its application method, in particular to a nucleic acid analysis collection device and its application method. (2) Background technology [0002] In recent years, with the rapid and in-depth development of molecular biology and the completion of the human genome framework, in the field of life science research, people have turned their research focus to the so-called "post-genome engineering". The project focuses more on the research of functional genes, especially the relationship between the transcription and expression of various functional genes. It is helpful for people to decipher the "holy book" of the genome framework diagram, understand life phenomena from the mechanism, and reveal and grasp the key to life development. Natural laws, thereby optimizing and transforming organisms, controlling and treating diseases, play a vital role. [0003] To achieve the above goals, one of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 国前于金明廖湘鲁李敏王兴武魏玲郑燕
Owner SHANDONG TUMOR HOSPITAL
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