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Method for measuring casein iodide in fodder

A method of determination, iodized casein technology, applied in the field of determination of iodized casein, can solve the problems of qualitative instability and difficult control of individual differences, and achieve the effects of small relative deviation, low cost and simple operation

Inactive Publication Date: 2007-06-06
SHANGHAI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the former two are biological tests in vivo, individual differences are difficult to control, and they can only be qualitative and unstable. The latter require the use of expensive and complex instruments, complex pre-treatment of samples, and professional technicians, which are difficult to control. The status quo of widespread abuse of such hormones

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  • Method for measuring casein iodide in fodder
  • Method for measuring casein iodide in fodder

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Embodiment 1

[0035] Embodiment one: now specific embodiment is described as follows, and its concrete steps are:

[0036] 1. Drawing of working curve: Dilute the 1mg / ml iodine standard configured above to 0.05μg / ml, 0.1μg / ml, 0.2μg / ml, 0.3μg / ml, 0.4μg / ml, 0.5μg / ml ml200μl standard solution, take 0.2ml of it and place it in six 10ml graduated colorimetric tubes, then add 1.68ml of 0.01mol / l arsenous acid, 4ml of 2.5mol / l sulfuric acid and 4ml of water in sequence, mix well, and Incubate at 30°C for 15 minutes, and at the same time, bathe with 0.002mol / L ceric ammonium sulfate solution at the same temperature. After 15 minutes, quickly add 0.78ml of ceric ammonium sulfate solution to each colorimetric tube in turn, add water to the mark and mix well , control the reaction time in the water bath to 20 minutes, and measure the absorbance of each solution in the colorimetric tube sequentially at a wavelength of 317nm with water as a reference, denoted as At; at the same time, measure with disti...

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Abstract

The present invention relates to the measuring method of casein iodide in feed. The measuring method includes the following steps: drawing the standard curve of iodine ion catalyzed cerium-ammonium reaction, preparing feed sample and measuring of casein iodide in feed. During preparing the feed sample, the feed sample is treated through Soxlet's extraction, isoelectric point deposition and trichloroacetic acid deposition to eliminate interferential organic and inorganic iodide and make iodine in the sample exist only in protein iodide form. Therefore, the method has high protein iodide recovering rate, small error, simple operation, low cost and high sensitivity.

Description

technical field [0001] The invention relates to a method for measuring iodized casein, in particular to a method for measuring trace amount of iodized casein in feed by adopting catalytic kinetic spectrophotometry. Background technique [0002] Thyroxine is an important hormone secreted by the thyroid gland of animals. Its physiological function is to comprehensively improve basic metabolism. It plays an extensive and important role in regulating the metabolism of the three major substances in the body and maintaining the normal growth and energy metabolism of the body. Iodized casein is a synthetic protein anabolic hormone, which is the precursor substance of thyroxine T3 and T4, and has similar physiological functions to T3 and T4 after being digested and absorbed by the intestinal tract. The product was first successfully developed by the U.S. Agricultural Technology Development Corporation. It was once approved by the U.S. Drug and Food Administration (FDA) as a feed add...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/28
Inventor 陈宇光胡建军沈彦萍
Owner SHANGHAI UNIV
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