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Preparation method of anti prglet bacterial epidemic disease compound egg antibody medicinal preparation

A yolk antibody, bacterial technology, applied in the direction of antiviral agents, antibodies, etc., to achieve the effect of no side effects, quick effect, and low cost

Inactive Publication Date: 2007-01-31
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] After searching the literature of the prior art, it was found that the Chinese invention patent application number: 03117507, the name of the invention: the yolk antibody against the pathogenic bacteria of yellow scour and pullorum and its preparation and application, the publication number: 1438246, the patent has developed an anti-yellow scour of piglets , the yolk antibody of pullorum pathogenic bacteria, but the yolk antibody needs to be purified before use, and it is a single preparation

Method used

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Embodiment Construction

[0009] Embodiments of the invention are provided below:

[0010] Raise clean-grade laying hens, and immunize E. coli K for the first time 88 、K 99 , 987P polyvalent genetically engineered vaccine and a self-made Salmonella vaccine. The second immunization was carried out at an interval of 10 days, and the immunization dose was 2 doses. The third immunization was carried out 10 days after immunization, and the immunization dose was 4 heads. 10 days after the end of the third immunization, after the titer of egg yolk antibody was determined (the plate agglutination test titer reached 1:40 or more), the immunized eggs were collected, and the egg yolk was aseptically obtained, diluted with 0.01M sterile phosphate buffer, 0.5% Hydroxypropyl methylcellulose phthalate is treated, the suction filtrate is spray-dried, and the powder is packaged and preserved. The yield of IgY is more than 90%.

[0011] For the diarrhea caused by the disease in piglets, the product of the present i...

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Abstract

A compound yolk antibody preparation for preventing bacterial disease of piglet, especially the bacteria diarrhea, is prepared through preparing salmonella vaccine and the genetically engineered vaccine of colibacillus K88, K99 and 9879, forced immunizing three times, collecting immunized egg, taking yolk, diluting with aseptic buffer liquid of phosphate, treating by hydroxypropylmethyl cellulose phthalate, filtering and spray drying.

Description

technical field [0001] The invention relates to a preparation method of an antibody preparation in the field of biotechnology, in particular to a preparation method of a composite egg yolk antibody preparation against piglet bacterial disease. Background technique [0002] Enterotoxic Escherichia coli (ETEC) and Salmonella are the main pathogens of diarrhea-based intestinal bacterial infectious diseases in piglets. Features acute onset, widespread prevalence, high mortality, seriously hindering the development of pig industry. In production, Escherichia coli polyvalent genetically engineered vaccine and Salmonella vaccine are mainly used to immunize multiparous sows, and protect piglets from damage through colostrum, but the effect is poor. Antibiotics are used in the treatment, which can easily destroy the normal flora of the digestive tract or produce drug-resistant strains. At the same time, because the main causative factor is enterotoxin, the treatment effect is not go...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/395A61P31/12
Inventor 徐建雄刘永德叶陈梁
Owner SHANGHAI JIAO TONG UNIV
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