Debilitating agent for highly toxic chicken coccidian and its preparation

Abstract

The invention relates to a poisonous chicken coccidia weakening agent and a preparation method, which is prepared from the following material components: potassium dichromate, sodium chloride, sodium bicarbonate, glucose, potassium chloride , sodium azide, distilled water. The attenuating agent of the present invention can be used to weaken various virulent strains of chicken coccidia such as tender, heap type, giant, poisonous and Eimeria precocci etc., and it is safe and reliable to inoculate chicks with the weak coccidia vaccine of the present invention, and Has good immunogenicity. The production facility of the present invention is simple, the cost is low, and the production investment of chicken coccidiosis vaccine can be reduced, the cycle is fast, the method is simple and practical, and it is convenient for popularization and application in rural areas. The cost is about 0.30 yuan / feather. After the control, the mortality rate of chickens is reduced, the egg production rate is increased, and the growth rate is accelerated.

Description

Technical field [0001] The invention relates to the preparation of a chicken coccidiosis vaccine, specifically, a virulent chicken coccidiosis attenuator and a preparation method thereof Background technique [0002] Chicken coccidiosis is a very harmful intestinal parasitic protozoan disease, which is mainly caused by Eimeria coccidiosis in intestinal epithelial cells. The disease is characterized by hemorrhagic enteritis, bloody dysentery, and high morbidity and mortality of chicks. Sometimes the infection rate of chicks is as high as 80%, and the mortality rate can reach 20%-50%. It has become one of the important diseases that endanger the chicken industry. At present, the prevention and treatment of the disease mainly includes drug control method and vaccine control method, wherein drug control of coccidiosis mainly achieves the effect of preventing and treating coccidiosis by mixing preventive or therapeutic doses of anticoccidial drugs in feed or water. Commonly used...

AI Technical Summary

Problems solved by technology

However, this kind of virulent insect seedling has not been widely used for more than 40 years. The distribution of oocysts in drinking water or feed is uneven, resulting in inconsistencies in the amount of oocysts ingested by chickens. As a result, some chickens are too lightly infected to produce immunity, and some chickens ingest too much oocysts and cause disease

However, the attenuation method of chicken embryo subculture has four disadvantages: ① the attenuation performance is relatively unstable, and the virulence returns to strong quickly when it is transferred to chicken; ② it is difficult to produce a large number of oocysts of commercial attenuated strains through chicken embryos; ③ As the number of passages of chicken embryos increases, the immunogenicity of the parasites will be reduced or lost. In fact, it is difficult to obtain the best combination that maintains good immunity and makes the virulence weaker; ④The main limitation is that the most popular Although the broad giant, pile type and Eimeria precocci can develop in chicken embryos, the growth of oocysts is poor, so the production of "full price" attenuated larvae through chicken embryo subculture is limited

Progress has been made in the understanding of the basic nature of coccidia infection and immunity by using genetic engineering technology, but it is still far away from the goal of making genetically engineered vaccines, and it will take time to transfer to the practical stage