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Pre-and posharvest inhibition of remobilisation of storage compounds

A compound, postharvest technology, applied in the field of inhibition of retransfer of preharvest and postharvest storage compounds, able to solve problems such as germination

Inactive Publication Date: 2001-01-17
杰尼克莫根有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Potatoes can be stored at higher temperatures to prevent cold sweetening, but this will cause them to sprout, which is undesirable

Method used

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  • Pre-and posharvest inhibition of remobilisation of storage compounds

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Experimental program
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Effect test

Embodiment 1

[0058] In one part of the experiments, tuber material was generated from in vitro transgenic pMOG845(patatin-tps) potato plants. A field trial was designed with 9 independent transgenic lines, 3 plots per line, and 5 tubers per plot. Tubers were planted in early May, and the germination process was monitored using conventional methods. The results are shown in Table 1. In the second part of the experiment, the pat-TPS plants (var. Kardal) from the tissue culture plants were placed in the plant incubator, 500 μmol light quantum m-2s-1 (16 hours of light, 20_C; 8 hours of darkness (15_C)) . The tubers were harvested after 3 months and stored at low temperature (4_C) for 2 months. Then move to room temperature (RT) and assess germination over 4 weeks.

[0059] plant strain

[0060] Tubers that did not sprout at all have been shown to have higher levels of transgene expression. The reduction in cold-sweetening described in WO 97 / 42326 was observed in non-germinating...

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PUM

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Abstract

This invention describes a method to prevent sprouting in vegetatively propagated plants such as potato, strawberry, banana and bulbous plants such as onion and bulbous flowers, by transforming a plant or a plant from one of its parental lines with a gene coding for trehalose phosphate synthase. Restoration of sprouting is also provided for.

Description

field of invention [0001] This application relates to the inhibition of retransfer of preharvest and postharvest storage compounds and in particular describes the inhibition of germination following transformation of vegetatively propagated plants with recombinant DNA and a method of restoring germination in these plants. Background of the invention [0002] As with agricultural genetic engineering, the main purpose of conventional breeding is to obtain high-yield crops, which usually means that the purpose of breeding is to increase the amount of storage in crop storage organs, such as potato tubers, sugar beet taproots, leaves of leafy crops such as lettuce. quantity. However, some other process in the plant, such as flowering and / or germination, often affects yield. [0003] Refrigerating at very low temperatures (slightly above freezing) can often suppress sprouting. Refrigeration is not only expensive, but also has a destructive effect on the storage organ, hindering ...

Claims

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Application Information

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IPC IPC(8): A01H1/00A01H5/00C12N9/10C12N9/16C12N15/09C12N15/31C12N15/54C12N15/55C12N15/82C12N15/84
CPCC12N15/8245C12N9/1051C12N15/8261C12N15/8267C12N9/16Y02A40/146
Inventor O·J·M·古德迪恩H·迪格拉尔K-P·克劳斯C·M·P·范邓恩
Owner 杰尼克莫根有限公司
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