High accuracy rate biologic enzyme blood type diagnosis reagent and preparation method thereof

Abstract

The invention relates to a high-accuracy blood type diagnosing reagent by blood type serological immune reaction, specially a blood type diagnosing reagent of Rh, Kidd and so on and cross-mixed blood as well as preparing method. The diagnosing reagent includes pawpaw proteinase powder and enzyme inhibitor, pawpaw proteinase diluted solution and enzyme inhibitor diluted solution, and the preparing method: firstly cultivate streptomycin by activated culture medium, then enlarge culture, and finally make industrialized production in fermentation cylinder, and then obtain the finished product through inactivation, purification, condensation and drying. Compared with other medium reagents, the diagnosing agent has characters of higher detecting sensitivity, accurate and reliable identification result, simple method, etc, applied in vitro blood type serological diagnosis tests such as antibody screening test, antibody identification test, antibody effect titration test, transfusion compatibility test, and sensitive blood type identification test.

Description

Technical field [0001] The invention relates to a blood group serological immune reaction test diagnostic reagent and a preparation method thereof. Especially the method of using reagents such as papain in the preparation of Rh, Kidd and other blood type and cross-matching diagnostic reagents. Background technique [0002] When the blood types of the general population are classified by the main blood type of ABO, most of them coexist with Rh blood group antigens or blood group antibodies. As the population gradually becomes urbanized, medical treatment, childbirth, and population mobility will make routine blood group experiments and cross-matching The number of experimenters has increased significantly, especially for Rh blood group antigens or blood group antibodies, which must be strictly diagnosed to ensure the safety of blood transfusion, marriage, and childbirth. At present, in developed countries in basic medical research abroad, the immunological diagnosis method for the...

AI Technical Summary

Problems solved by technology

[0005] However, the use of papain to make blood type and cross-match diagnostic reagents also has some shortcomings, because papain is extracted from natural plants, so there are inherent defects in natural enzymes, which are mainly manifested in different batches of products from different manufacturers. During the separation, purification, reaction, preparation and application of the production process, the activity of the enzyme varies, and the activity is unstable, so the reaction speed is not uniform, and the concentration of the reaction product is also different. In addition, most natural enzymes are harmful to the human body. Allogeneic substance, the enzyme itself has antigenicity, as a protein, the enzyme is easy to be affected by some human proteins, inhibitors and antibodies to affect the activity of the enzyme, which will also affect the real results of the reaction experiment, the more important defect It is a "false positive" result of non-specific agglutination due to the activation of antigens caused by the hydrolysis of the surface of blood red blood cell membrane proteins by papain and other diagnostic reagents. On the other hand, the reagents degrade the blood group antibodies when they are added into the experiment. "false negative" results