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Strain producing remarkable amount of epsilon-poly-L-Lysine and process for producing same

A lysine, strain technology, applied in bacteria, fungi, fermentation and other directions, can solve problems such as unsatisfactory

Inactive Publication Date: 2000-07-12
JNC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To produce inexpensive εPL that can be used for various purposes, even the use of the above-mentioned 11011A-1 strain is not satisfactory in terms of εPL yield and glucose conversion efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] In a container with a volume of 3 liters, fill 2 liters of medium consisting of: glucose: 5%, ammonium sulfate: 1%, yeast extract: 0.5%, K 2 HPO 4 : 0.08%, KH 2 PO 4 : 0.136%, MgSO 4 ·7H 2 O: 0.05%, ZnSO 4 ·7H 2 O: 0.004%, FeSO 4 ·7H 2 O: 0.003%, pH 6.8. Inoculate 100 milliliters of Streptomyces albicans lysinopolymerus subspecies B21021 bacterial strain, namely the preculture of the improved bacterial strain of the present invention, into the culture medium, cultivate aerobically at 30° C. at 700 rpm for 72 hours, and the air flow rate is 3 L / min. The pH was maintained at 4 with 10% aqueous ammonia. When the concentration of residual glucose and ammonium sulfate decreases, add glucose and ammonium sulfate continuously.

[0074] Table 3 shows the yield of εPL and the conversion rate of glucose after 72 hours of cultivation. "Glucose conversion (%)" is expressed herein as (εPL production / glucose consumption) x 100.

Embodiment 2

[0076] Except for using Streptomyces albicans subsp. lysinopolymerus B22107 strain instead of Streptomyces albicans subsp. lysinopolymerus B21021 strain, culture was carried out as in Example 1, and the amount of εPL in the culture was measured. Table 3 shows the production of εPL and the conversion rate of glucose after 72 hours of cultivation.

Embodiment 3

[0078] Except for using Streptomyces albicans subspecies lysinopolymerus B22201 strain instead of Streptomyces albicans subsp. lysinopolymerus B21021 strain, culture was carried out as in Example 1, and the amount of εPL in the culture was measured. Table 3 shows the production of εPL and the conversion rate of glucose after 72 hours of cultivation.

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Abstract

A strain which has an enhanced ability to produce 'epsilon'-poly-L-Lysine as compared with the conventional 'epsilon'-poly-L-Lysine-producing strains or improved variesties thereof, and is capable ofelevating 'epsilon'-poly-L-Lysine productivity; and a process for producing a remarkable amount of 'episilon'-poly-L-Lysine by using this strain which comprises mutagenizing a microorganism capable of producing 'epsilon'-poly-L-Lysine and belonging to the species Streptomyces albulus to thereby give a strain being tolerant to S-(2-aminoethyl)-L-cysteine at a high concentration of 10 mg / ml or above and caapble of producing 'epsilon'-poly-L-Lysine, aerobically incubating this strain in a medium, and collecting the 'epsilon'-poly-L-Lysine from the culture.

Description

field of invention [0001] The present invention relates to a bacterial strain producing ε-poly-L-lysine (hereinafter referred to as εPL) in large quantities and a method for producing εPL by fermentation using the bacterial strain. [0002] Since εPL is a polymer of the essential amino acid L-lysine, it is safe and has unique physical properties due to its high cationic content. Therefore, it is expected to be used in cosmetics, beauty products, feed additives, pharmaceuticals, insecticides, food additives, electronic equipment and the like. Especially in the field of food additives, it has drawn attention as a natural additive. Background technique [0003] As a method for fermentatively producing εPL, there is known a method (approved Japanese Patent Laid-Open No. 59-20359), which comprises culturing Streptomyces parvus lysinopolymerus subspecies 346-D (FERM P-3834) in a culture medium, which strain is The εPL-producing strain isolated from nature, belonging to the genus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20C12P13/12C12P21/00
CPCC12P21/00
Inventor 岩田敏治白石慎治岩泽由美子平木纯
Owner JNC CORP
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